AI Article Synopsis
- Carpesii Fructus, a dried fruit used in traditional Chinese medicine to treat intestinal parasites, has been shown to have developmental toxicity in zebrafish embryos/larvae for the first time.
- Common morphological changes observed in treated zebrafish included pericardial and yolk sac edema, bleeding tendencies, and enlarged yolk, indicating potential heart vulnerability.
- The study found alterations in enzyme activity and gene expression linked to oxidative stress and apoptosis, suggesting these mechanisms contribute to the observed developmental toxicity of Carpesii Fructus.
Article Abstract
Carpesii Fructus, the dried fruit of L., has been used as a traditional Chinese medicine for centuries to kill intestinal parasites in children. It has been recorded as a mildly toxic medicine in the Chinese pharmacopoeia. However, little proof of its toxicology has been reported in modern pharmacology. This study investigated for the first time its developmental toxicity on zebrafish embryos/larvae from 6 to 96 h post-fertilization (hpf). In addition, the enzymes and genes associated with oxidative stress and apoptosis were tested to investigate the potential toxicologic mechanism preliminarily. The observation of toxicologic endpoints showed the developmental toxicity of Carpesii Fructus. Pericardial edema, yolk sac edema, bleeding tendency, and enlarged yolk were the most commonly occurring morphological changes observed in our study. According to the results of acridine orange staining and morphological observation, the developing heart was speculated to be the target organ of toxicity. Furthermore, Carpesii Fructus exposure changed the activities of defense enzymes, increased malondialdehyde (MDA) content, decreased caspase-3 activity, and altered mRNA levels of related genes (o, , /, , and ↓↑) in zebrafish larvae, indicating that oxidative stress and additional apoptosis should have roles in the developmental toxicity of Carpesii Fructus. This is the first study that provides proof of modern pharmacology on the teratogenicity and possible toxicologic mechanism of Carpesii Fructus.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6062322 | PMC |
| http://dx.doi.org/10.1039/c7tx00005g | DOI Listing |
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Article Synopsis
- Carpesii Fructus, a dried fruit used in traditional Chinese medicine to treat intestinal parasites, has been shown to have developmental toxicity in zebrafish embryos/larvae for the first time.
- Common morphological changes observed in treated zebrafish included pericardial and yolk sac edema, bleeding tendencies, and enlarged yolk, indicating potential heart vulnerability.
- The study found alterations in enzyme activity and gene expression linked to oxidative stress and apoptosis, suggesting these mechanisms contribute to the observed developmental toxicity of Carpesii Fructus.
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