AI Article Synopsis
- - The Malayan pangolin primarily eats ants and termites, which are rich in chitin, a carbohydrate that can be digested with specific enzymes in its stomach.
- - The research aimed to check the presence of acidic mammalian chitinase (AMCase) in different parts of the pangolin's stomach using techniques like RT-PCR and western blot.
- - Results showed that AMCase is highly expressed in the oxyntic glands of the Malayan pangolin, indicating its important role in digesting chitin-rich food.
Article Abstract
The Malayan pangolin () is a mammal that feeds primarily on ants and termites, which contain the energy-rich carbohydrate chitin. Chitin is digestible by endogenous enzymes of the typical mammalian gastrointestinal tract, especially the acidic mammalian chitinase (AMCase). The objective of this research was to determine whether AMCase activity is expressed in the stomach of . The stomach tissues were divided into three parts: the gastric sack, the oxyntic glands, and the pyloric musculature, which were assayed by conventional RT-PCR, quantitative reverse transcriptase-coupled PCR (qPCR) and western blot. Information regarding 3D structural models of AMCase was also obtained. In conclusion, acidic mammalian chitinase is highly expressed in the oxyntic glands of the species.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070644 | PMC |
| http://dx.doi.org/10.1002/2211-5463.12461 | DOI Listing |
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