AI Article Synopsis
- Developed a shuttle vector, pSCB-YFP, for the cyanobacterium PCC6803, featuring a replicon from pCC5.2 and a YFP reporter gene.
- The vector is stably maintained in a motile strain lacking endogenous pCC5.2, enabling the expression of YFP.
- It includes multiple cloning sites, functions as an expression vector, and remains stable for over 50 generations without antibiotic selection.
Article Abstract
To advance synthetic biology in the photosynthetic cyanobacterium sp. PCC6803 (Syn6803), we constructed a shuttle vector with some versatile features. This shuttle vector, pSCB-YFP, consists of a putative replicon identified on the plasmid pCC5.2, the origin of replication of pMB1 from , as well as the YFP reporter gene and a spectinomycin/streptomycin resistance cassette. pSCB-YFP is stably maintained in Syn6803M (a motile strain that lacks the endogenous pCC5.2) and expresses YFP. In addition, we engineered a fragment into pSCB-YFP that has multiple cloning sites and other features such that this plasmid can also be used as an expression vector (pSCBe). The shuttle vector pSCB-YFP can be stably maintained for at least 50 generations without antibiotic selection. It is a high copy number plasmid and can stably co-exist with the RSF1010-based pPMQAK1-GFP.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066503 | PMC |
| http://dx.doi.org/10.3389/fmicb.2018.01662 | DOI Listing |
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