OXA-535 is a chromosome-encoded carbapenemase of JAB-1 that shares only 91.3% amino acid sequence identity with OXA-48. Catalytic efficiencies are similar to those of OXA-48 for most β-lactams, except for ertapenem, where a 2,000-fold-higher efficiency was observed with OXA-535. OXA-535 and OXA-436, a plasmid-encoded variant of OXA-535 differing by three amino acids, form a novel cluster of distantly related OXA-48-like carbapenemases. Comparison of and genetic environments suggests that an IS may be responsible for gene mobilization from the chromosome of spp. to plasmids.
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http://dx.doi.org/10.1128/AAC.01198-18 | DOI Listing |
J Mol Diagn
February 2020
EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Université Paris-Sud, Université Paris-Saclay, LabEx Laboratory of Excellence in Drug Discovery and Innovative Therapeutics (LERMIT), Faculty of Medicine, Le Kremlin-Bicêtre, France; Evolution and Ecology of Resistance to Antibiotics Unit, Institut Pasteur, Assistance Publique-Hôpitaux de Paris, Université Paris-Sud, Paris, France; Bacteriology-Hygiene Unit, Bicêtre Hospital, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre, France; Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France. Electronic address:
A novel real-time multiplex PCR assay, BD MAX Check-Points CPO, was evaluated to detect carbapenemase-producing organisms in clinical settings on the BD MAX system. A total of 175 well-characterized isolates (including 123 carbapenemase producers) and 128 rectal swab specimens (including 83 positives) of patients considered at high risk for carriage of carbapenemase producers were included. Bacterial suspensions were used to spike true-negative rectal swabs to mimic a clinical sample.
View Article and Find Full Text PDFAntimicrob Agents Chemother
October 2018
EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases, " Paris-Sud University, Faculty of Medicine, Le Kremlin-Bicêtre, France
OXA-535 is a chromosome-encoded carbapenemase of JAB-1 that shares only 91.3% amino acid sequence identity with OXA-48. Catalytic efficiencies are similar to those of OXA-48 for most β-lactams, except for ertapenem, where a 2,000-fold-higher efficiency was observed with OXA-535.
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