Eleven primer pairs were developed for the identification of . The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 isolates and 40 other or non- species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50 cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for diagnosis.
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| http://dx.doi.org/10.3390/antibiotics7030070 | DOI Listing |
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