AI Article Synopsis
- Recent changes in PRRS virus strains have led to some being isolated only from PAMs and not from Marc-145 cells.
- Researchers isolated these PRRSV strains and found that they belong to specific lineages (HP-PRRSV and NADC30-Like).
- The study also revealed that variations in certain proteins are key to understanding how the virus adapts to different cells, particularly the Marc-145 cells.
Article Abstract
The recent rapid evolution of PRRSVs has resulted in certain biological characteristic changes, such as the fact that an increasing number of field PRRSVs can be isolated from PAMs but not from Marc-145 cells. In this study, we first isolated Marc-145-unadaptive field PRRSV strains from PAMs; sequence analysis showed that these PRRSVs belong to the HP-PRRSV (lineage 8) branch or NADC30-Like (lineage 1) branch. We further found major variations in ORF2-4 regions. To explore the viral adaptation mechanisms in detail, we constructed a full-length cDNA clone of MY-376, a Marc-145-unadaptive PRRSV. Construction of serially chimeric viruses of HuN4-F112 (a Marc-145-adaptive strain) and MY-376 demonstrated that variation in the minor envelope protein (GP2a and GP3) complex is a main determinant of PRRSV tropism for Marc-145 cells.
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| http://dx.doi.org/10.1016/j.vetmic.2018.06.021 | DOI Listing |
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