In previous work we have shown that recycling pre-sonicated drinking water treatment sludge (DWTS) could improve coagulated water quality. Here, the removal of naturally occurring organic matter of source water was further optimized using response surface methodology (RSM) with Box-Behnken Design (BBD). The four variables, i.e., volumetric recycling ratio of DWTS, energy density, ultra-sonication time and duty cycle in an experimental jar test of ultrasound assisted flocculation-coagulation were optimized. All the variables showed a significant effect on dissolved organic carbon (DOC) removal of source water (p < .05), of which the duty cycle had a stronger effect on the removal performance compared to the other independent variables. The predicted optimal DOC removal rate was 36.94%, and this matched well the observed performance of 36.54 ± 0.56%, obtained by ultra-sonicating the sludge prior to recycling using a power input of 1.015 W/mL, an ultra-sonication time of 9 min 50 s, and a duty cycle of 80%, while the volumetric recycling ratio of DWTS was 5.8%. The natural organic matter fractions in the coagulated water samples indicated that recycling sonicated DWTS that had been washed prior to recycling in order to remove solubilized extracellular polymers could enhance removal of hydrophobic acids and 3-30 kDa fractions, but this treatment increased the presence of substances with molecular weight <3 kDa. Humic-like substances were effectively removed while tyrosine-like substances could be enriched. Sludge samples (raw DWTS, sonicated DWTS, sludge formed by recycling raw DWTS, and sludge formed by recycling sonicated DWTS without solubilized extracellular organics) were characterized by XRF, X-ray diffraction patterns and FE-SEM-EDS to reveal possible physical characteristics that could be related to the DOC removal performance.
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