Objective: To study the chemical consituents of Hypericum ascyron.

Methods: The constituents were isolated and purified by chromatography on silica gel; the structure of the compound was determined by MS and NMR spectral analysis.

Results: On the basis of spectroscopic analysis and comparison with the reported data, they were identified as hyperoside( 1),hypercalin B( 2),hypercalin C( 3),1,7-dihydroxyxanthone( 4),2,3-dimethoxyxanthone( 5),1-hydroxy-7-methoxyxanthone( 6),rutin( 7),kaempferol( 8),toxyloxanthone B( 9),quercetin( 10),quercitrin( 11),β-daucosterol( 12) and β-sitosterol( 13).

Conclusion: Compounds 2,3,6 and 9 are obtained from this plant for the first time.

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