[Cloning,Analysis and Expression on Gene of Squalene Synthase in Citrus reticulata Seeds].

Objective: To clone squalene synthase gene( ss) from seeds of Citrus reticulate ‘Dahongpao’,and to analyze its expression and bioinformatics.

Methods: The technology of RT-PCR was used to clone the gene of Citrus reticulata seeds and analyze its expression at various growing stages. Moreover, the bioinformatics was analyzed by online analytical tools.

Results: The full-length of ss was1 205 bp and 397 amino acids were encoded, SS had a homology of 99% with that of Citrus sinensis and Citrus clementina. The growth process of Citrus reticulata seeds was divided into three stages: morphogenesis stage, maturity and dehydration periods, and expression of ss was the highest in maturity period.

Conclusion: Squalene synthase gene from seeds of Citrus reticulate ‘Dahongpa’is obtained for the first time, which provides reference for biosynthesis and regulation mechanism research of limonin compounds in Citrus reticulata seeds.