AI Article Synopsis
- The study explores the reconstitution of ribosomal subunits (30S) in vitro using purified ribosomal proteins and ribosome biogenesis factors, offering insights into ribosome assembly.
- Unlike traditional methods that require high-salt conditions, this new approach allows assembly under physiological conditions, enabling the synthesis of proteins.
- The findings provide a platform for further research on ribosome assembly and function, and the potential to create ribosomes directly from DNA without the need for living cells.
Article Abstract
Reconstitution of ribosomes in vitro from individual ribosomal proteins provides a powerful tool for understanding the ribosome assembly process including the sequential incorporation of ribosomal proteins. However, conventional assembly methods require high-salt conditions for efficient ribosome assembly. In this study, we reconstituted 30S ribosomal subunits from individually purified ribosomal proteins in the presence of ribosome biogenesis factors. In this system, two GTPases (Era and YjeQ) facilitated assembly of a 30S subunit exhibiting poly(U)-directed polyphenylalanine synthesis and native protein synthesis under physiological conditions. This in vitro system permits a study of the assembly process and function of ribosome biogenesis factors, and it will facilitate the generation of ribosomes from DNA without using cells.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191716 | PMC |
| http://dx.doi.org/10.1261/rna.065615.118 | DOI Listing |
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