AI Article Synopsis
- This study investigates the role of the Sortase A (srtA) gene in the coaggregation and co-adherence of Streptococcus mutans with various salivary bacteria.
- Coaggregation assays showed that both the S. mutans UA159 strain and its srtA-deficient mutant bound to Fusobacterium nucleatum, but the mutant had lower co-adherence levels.
- Overall, the deletion of the srtA gene reduces S. mutans' ability to bind specifically to F. nucleatum without significantly changing its co-adherence to other salivary bacteria.
Article Abstract
This study aimed to study whether the Sortase A (srtA) gene helps mediate coaggregation and co-adherence between Streptococcus mutans (S. mutans) and other salivary bacteria. S. mutans UA159 and srtA-deficient mutant served as "bait" in classical co-aggregation assays and membrane-based co-adherence assays were used to examine interactions of S. mutans with Fusobacterium nucleatum (F. nucleatum), Streptococcus mitis (S. mitis), Streptococcus gordonii (S. gordonii), Streptococcus sanguis (S. sanguis), Actinomyces naeslundii (A. naeslundii) and Lactobacillus. Co-adherence assays were also performed using unfractionated saliva from healthy individuals. Co-adhering partners of S. mutans were sensitively detected using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Both UA159 and its srtA-deficient mutant bound to F. nucleatum but not to any of the other five salivary bacteria. The srtA-deficient mutant showed lower co-adherence with F.nucleatum. The two S. mutans strains also showed similar co-adherence profiles against unfractionated salivary bacteria, except that UA159 S. mutans but not the srtA-deficient bound to a Neisseria sp. under the same conditions. Deleting srtA reduces the ability of S. mutans to bind to F.nucleatum, but it does not appear to significantly affect the binding profile of S. mutans to bulk salivary bacteria.
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| http://dx.doi.org/10.1007/s11596-018-1860-y | DOI Listing |
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