Characterization of a green nanocomposite prepared from soluble soy bean polysaccharide/Cloisite 30B and evaluation of its toxicity.

The present paper aims to elucidate the structural, thermal and mechanical properties of soybean polysaccharide (SSPS)/Cloisite 30B. Tensile strength of the nanocomposite films improved with incorporation of nanoparticles, whereas elongation at break decreased. Surface roughness of the samples increased with the addition of nanoclay. Neat SSPS film and SSPS-1% Cloisite 30B had a relatively smooth surface with no irregularities, while for the samples containing 3 and 7% Cloisite 30B, the surface was rough. DSC analysis demonstrated that following an increase in nanoparticles content, the melting temperature of the nanocomposite elevated, whereas, glass transition temperature decreased. The results of antibacterial activity indicated that Cloisite 30B could inhibit the growth of Salmonella typhi PTCC 1609, Staphylococcus epidermis PTCC 1114 (ATCC 12228) and Listeria monocytogenes PTCC 1165. SSPS-Cloisite 30B nanocomposite could not inhibit the growth of Aspergillus niger. The results demonstrated that the migration of nanoparticles might happen into deionized water as a food simulant, but they could not migrate into bread as a food model. Furthermore, it was found that Cloisite 30B nanoparticles had cytotoxicity effect, and thus, it is recommended that Cloisite 30B/SSPS nanocomposites be used only for the packaging of solids foods such as bread.