AFM and FluidFM Technologies: Recent Applications in Molecular and Cellular Biology.

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Materials, Natural Substances, Environment and Modeling Laboratory, Multidisciplinary Faculty of Taza, University Sidi Mohammed Ben Abdellah, Fez, Morocco.

Published: November 2018

AI Article Synopsis

  • Atomic force microscopy (AFM) is a popular imaging method in material sciences that has also been adapted for biological applications like studying cell organelles and DNA-protein interactions.
  • Recent advancements, such as fluidic force microscopy (FluidFM), enhance AFM by allowing for precise liquid dispensing, which addresses challenges in analyzing single cells.
  • This review focuses on recent studies exploring the use of AFM in molecular and cellular biology, highlighting its versatility and impact on scientific research.

Article Abstract

Atomic force microscopy (AFM) is a widely used imaging technique in material sciences. After becoming a standard surface-imaging tool, AFM has been proven to be useful in addressing several biological issues such as the characterization of cell organelles, quantification of DNA-protein interactions, cell adhesion forces, and electromechanical properties of living cells. AFM technique has undergone many successful improvements since its invention, including fluidic force microscopy (FluidFM), which combines conventional AFM with microchanneled cantilevers for local liquid dispensing. This technology permitted to overcome challenges linked to single-cell analyses. Indeed, FluidFM allows isolation and injection of single cells, force-controlled patch clamping of beating cardiac cells, serial weighting of micro-objects, and single-cell extraction for molecular analyses. This work aims to review the recent studies of AFM implementation in molecular and cellular biology.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057332PMC
http://dx.doi.org/10.1155/2018/7801274DOI Listing

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