2-Aminoacetophneone (2-AA) is a volatile molecule produced in high amounts by the opportunistic pathogen Pseudomonas aeruginosa. We have previously shown that 2-AA activates the quorum sensing (QS) LuxR receptor of Aliivibrio fischeri. In the present study we were able to improve LuxR's affinity and detection limit for 2-AA by genetic modification of three amino acids within the binding pocket of the receptor. Expression of the modified LuxR receptor in a luminescent bacterial biosensor provided an efficient detection assay of 2-AA in clinical P. aeruginosa strains isolated from blood and lung infections, as well as in phlegm samples obtained from subjects suffering from lung infections.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c8cc03540g | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Unlabelled: is a Gram-negative opportunistic pathogen that poses a significant public health threat, particularly in healthcare settings. A key determinant of virulence is the regulated synthesis and release of extracellular products, which is controlled by a cell density-dependent signaling system known as quorum sensing (QS). uses a complex QS network, including two systems that rely on diffusible N-acylhomoserine lactone (AHL) signal molecules.
View Article and Find Full Text PDFInterspecies Crosstalk via LuxI/LuxR-Type Quorum Sensing Pathways Contributes to Decreased Nematode Survival in Coinfections of and .
ACS Chem Biol
December 2024
Department of Chemistry, University of Wisconsin-Madison, 1101 University Ave., Madison, Wisconsin 53706, United States.
Quorum sensing (QS) is a prominent chemical communication mechanism used by common bacteria to regulate group behaviors at high cell density, including many processes important in pathogenesis. There is growing evidence that certain bacteria can use QS to sense not only themselves but also other species and that this crosstalk could alter collective behaviors. In the current study, we report the results of culture-based and coinfection experiments that probe interspecies interactions between the opportunistic pathogens and involving their LuxI/LuxR-type QS circuits.
View Article and Find Full Text PDFQuorum quenching activity of endophytic sp. EBS9 from and its biocontrol applications.
Curr Res Microb Sci
October 2024
Department of Microbiology and Biotechnology, IIS (Deemed to be University), Jaipur, Rajasthan, India.
This study investigates the quorum quenching (QQ) activity of an endophytic bacterium, sp. EBS9, isolated from the native medicinal plant of Rajasthan, and its biocontrol potential against the soft rot pathogen subsp. ().
View Article and Find Full Text PDFIdentification of new SdiA regulon members of , , and serovars Typhimurium and Typhi.
Microbiol Spectr
October 2024
Department of Microbiology, The Ohio State University, Columbus, Ohio, USA.
Article Synopsis
- - Bacteria like Typhimurium and Typhi utilize a method called quorum sensing to detect population density through small molecules, specifically acyl homoserine lactones (AHLs), despite lacking the ability to produce these signals themselves; they can sense them using a receptor called SdiA, engaging in behavior known as eavesdropping.
- - RNA-seq analysis reveals that even though Typhimurium and Typhi have different pathogenic strategies, their regulatory networks (regulons) related to AHL detection are largely conserved, with some overlap in gene functions observed when testing related genes between the two serovars.
- - Many genes regulated by AHLs in these bacteria are not well understood, and the overall significance
Small RNA GadY in enhances conjugation system of IncP-1 by targeting SdiA.
Front Cell Infect Microbiol
August 2024
The Second Clinical Medical College, Guangzhou University of Chinese Medicine, State Key Laboratory of Traditional Chinese Medicine Syndrome, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China.
Plasmid-mediated conjugation is a common mechanism for most bacteria to transfer antibiotic resistance genes (ARGs). The conjugative transfer of ARGs is emerging as a major threat to human beings. Although several transfer-related factors are known to regulate this process, small RNAs (sRNAs)-based regulatory roles remain to be clarified.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!