AI Article Synopsis
- This study examined the effects of 12 weeks of resistance exercise training (RET) on muscle protein levels and mitochondrial markers in college-aged males classified as high (HI) or low (LO) anabolic responders.
- Results showed that HI responders had significantly greater increases in muscle mass and thickness compared to LO responders, but both groups exhibited similar levels of myofibrillar and sarcoplasmic proteins before and after training.
- The increase in mitochondrial volume in HI responders may have contributed to their better muscle-building outcomes compared to LO responders, while a specific protein (myozenin-1) was up-regulated in the LO group after training.
Article Abstract
Background: We sought to examine how 12 weeks of resistance exercise training (RET) affected skeletal muscle myofibrillar and sarcoplasmic protein levels along with markers of mitochondrial physiology in high versus low anabolic responders.
Methods: Untrained college-aged males were classified as anabolic responders in the top 25th percentile (high-response cluster (HI); = 13, dual x-ray absorptiometry total body muscle mass change (Δ) = +3.1 ± 0.3 kg, Δ vastus lateralis (VL) thickness = +0.59 ± 0.05 cm, Δ muscle fiber cross sectional area = +1,426 ± 253 μm) and bottom 25th percentile (low-response cluster (LO); = 12, +1.1 ± 0.2 kg, +0.24 ± 0.07 cm, +5 ± 209 μm; < 0.001 for all Δ scores compared to HI). VL muscle prior to (PRE) and following RET (POST) was assayed for myofibrillar and sarcoplasmic protein concentrations, myosin and actin protein content, and markers of mitochondrial volume. Proteins related to myofibril formation, as well as whole lysate PGC1-α protein levels were assessed.
Results: Main effects of cluster (HI > LO, = 0.018, Cohen's = 0.737) and time (PRE > POST, = 0.037, Cohen's = -0.589) were observed for citrate synthase activity, although no significant interaction existed (LO PRE = 1.35 ± 0.07 mM/min/mg protein, LO POST = 1.12 ± 0.06, HI PRE = 1.53 ± 0.11, HI POST = 1.39 ± 0.10). POST myofibrillar myozenin-1 protein levels were up-regulated in the LO cluster (LO PRE = 0.96 ± 0.13 relative expression units, LO POST = 1.25 ± 0.16, HI PRE = 1.00 ± 0.11, HI POST = 0.85 ± 0.12; within-group LO increase = 0.025, Cohen's = 0.691). No interactions or main effects existed for other assayed markers.
Discussion: Our data suggest myofibrillar or sarcoplasmic protein concentrations do not differ between HI versus LO anabolic responders prior to or following a 12-week RET program. Greater mitochondrial volume in HI responders may have facilitated greater anabolism, and myofibril myozenin-1 protein levels may represent a biomarker that differentiates anabolic responses to RET. However, mechanistic research validating these hypotheses is needed.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065464 | PMC |
| http://dx.doi.org/10.7717/peerj.5338 | DOI Listing |
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