Broccoli sprouts are considered functional food as they are naturally enriched in glucoraphanin (GR) that is the biological precursor of the anticancer compound sulforaphane (SFN). Due to its health promoting value, also broccoli sprout juice is becoming very popular. The present study aimed to quantitatively assess the conversion of GR to its hydrolysis products, SFN and SFN-nitrile, during the juice preparation process. We demonstrated that SFN plus SFN-nitrile yield from glucoraphanin is quite low (≈25%) and that some SFN is lost during the juice preparation partially due to the spontaneous conversion to sulforaphane-amine or conjugation to GSH and proteins naturally present in the juice. Our results demonstrate that the detection of the sole SFN free form does not provide reliable information about the real concentration of this functional compound in the juice.
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http://dx.doi.org/10.1016/j.foodchem.2018.06.089 | DOI Listing |
J Insect Physiol
January 2025
Institute of Biosciences and Applications, National Centre for Scientific Research Demokritos, Athens, Greece.
Double-stranded RNA (dsRNA) mediated RNA interference (RNAi) is a tool in functional gene study and pest control. However, RNAi efficiency in Lepidoptera is low compared to the RNAi sensitive Coleoptera. Previous studies on RNAi in the silkworm Bombyx mori, the lepidopteran model insect, were performed by injection only.
View Article and Find Full Text PDFAnal Methods
January 2025
Department of Nutrition and Dietetics, Faculty of Health Sciences, Hasan Kalyoncu University, Gazi-antep, 27000, Turkey.
In the present study, a novel voltammetric sensor based on a boron-doped copper oxide/graphene (B-CuO-Gr) nanocomposite and molecularly imprinted polymer (MIP) was developed for the detection of paclobutrazol (PAC) in apple and orange juice samples. The B-CuO-Gr nanocomposite was prepared using sol-gel and calcination methods. After modifying glassy carbon electrodes with the B-CuO-Gr nanocomposite, PAC-imprinted electrodes were prepared in the presence of 100.
View Article and Find Full Text PDFNutrients
December 2024
Department of Nutrition and Dietetic Sciences, School of Health Sciences, Hellenic Mediterranean University, 72300 Sitia, Greece.
Background/objectives: Home isolation measures during the COVID-19 lockdown periods may have influenced individuals' lifestyles. The COVEAT study aimed to identify differences in children's and their parents' dietary behavior, children's body weight and parental body mass index (BMI) between two lockdown periods implemented in Greece.
Methods: In total, 61 participants (children 2-18 years and their parents) completed questionnaires about their lifestyle, body weight and height, and family socio-demographic data, during both lockdown periods (LDs) implemented in Greece (LD1 in March-May 2020; LD2 in December 2020-January 2021).
Foods
December 2024
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
A highly sensitive lateral flow immunoassay (LFIA) for imidacloprid, a widely used neonicotinoid insecticide, has been developed. The LFIA realizes the indirect coupling of anti-imidacloprid antibodies and gold nanoparticle (GNP) labels directly in the course of the assay. For this purpose, the common GNPs conjugate with anti-imidacloprid antibodies and are changed into a combination of non-modified, anti-imidacloprid antibodies, and the GNPs conjugate with anti-species antibodies.
View Article and Find Full Text PDFPolymers (Basel)
December 2024
Department of Prosthodontics, Faculty of Dentistry, University of Abant İzzet Baysal, 14030 Bolu, Turkey.
This study evaluated the color stability, surface roughness, and hardness of 3D-printed and heat-polymerized denture materials. A total of 90 samples were prepared, with equal numbers of 3D-printed and heat-polymerized disks. The initial hardness, surface roughness, and color values of the samples were measured.
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