Growth improvement and metabolic profiling of native and commercial Chlorella sorokiniana strains acclimatized in recycled agricultural wastewater.

Bioresour Technol

Research Center for Sustainable Process Technology, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.

Published: January 2018

This study investigated acclimation ability of native Chlorella sorokiniana (CS-N) and commercial Chlorella sorokiniana (CS-C) in palm oil mill effluent (POME), their metabolic profile and feasibility of effluent recycling for dilution purpose. Maximum specific growth rate, µ and lag time, λ of the microalgae were evaluated. Result shows both strains produced comparable growth in POME, with µ of 0.31 day and 0.30 day respectively, albeit longer λ by the CS-C. However, three cycles of acclimation was able to reduce λ from eight days to two days for CS-C. Metabolic profiling using principal component analysis (PCA) shows clear cluster of acclimatized strains to suggest better stress tolerance of CS-N. Finally, a remarkable µ of 0.57 day without lag phase was achieved using acclimatized CS-N in 40% POME concentration. Acclimation has successfully shortened the λ and dilution with final effluent was proved to be feasible for further improvement of the microalgae growth.

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http://dx.doi.org/10.1016/j.biortech.2017.09.195DOI Listing

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