Morphologic changes in cyclophosphamide (CY)-suppressed vs. control non-suppressed new-born rats infected i.c. with XJC13 strain of Junin virus were compared and the cells involved in CNS lesions were identified by the PAP technique. Fifty per cent of the control rats exhibited widespread cerebral necrosis vs. only 15% of the immunosuppressed animals. The first cells to reach Junin virus-infected CNS in controls were T lymphocytes, which destroyed viral antigen-laden target neurons and astrocytes. B lymphocytes and macrophages, presumably attracted by viral antigen and/or by lymphokines, made their appearance a day or two later. Activated macrophages phagocytosed necrotic cells and perhaps exerted a cytotoxic effect upon target neural cells, whereas the actual role of B lymphocytes requires further explanation. In CY-treated rats, cerebral lesions were smaller and the cellular exudate, though similar, proved much scantier than in controls. A similar extent of cerebellar necrosis was observed in both groups.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0165-5728(86)90112-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Visualization of Double-Stranded RNA Colocalizing With Pattern Recognition Receptors in Arenavirus Infected Cells.
Front Cell Infect Microbiol
August 2019
Department of Pathology and Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, United States.
An important step in the initiation of the innate immune response to virus infection is the recognition of non-self, viral RNA, including double-stranded RNA (dsRNA), by cytoplasmic pattern recognition receptors (PRRs). For many positive-sense RNA viruses and DNA viruses, the production of viral dsRNA, and the interaction of viral dsRNA and PRRs are well characterized. However, for negative-sense RNA viruses, viral dsRNA was thought to be produced at low to undetectable levels and PRR recognition of viral dsRNA is still largely unclear.
View Article and Find Full Text PDFDDX3 suppresses type I interferons and favors viral replication during Arenavirus infection.
PLoS Pathog
July 2018
Division of Biological Sciences, University of California San Diego, La Jolla, CA, United States of America.
Article Synopsis
- Several arenaviruses are linked to severe hemorrhagic fever in humans, prompting the need for effective countermeasures.
- Utilizing an unbiased proteomic approach, researchers identified DDX3, a RNA-helicase, as a key protein interacting with arenavirus nucleoprotein (NP).
- The study revealed that DDX3 deficiency hinders the replication of both Old and New World arenaviruses, suggesting it could be a promising target for new therapies against these dangerous viruses.
Evaluation of cell viability dyes in antiviral assays with RNA viruses that exhibit different cytopathogenic properties.
J Virol Methods
August 2017
Institute for Antiviral Research, Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322 USA.
Studies were conducted to determine the performance of four dyes in assessing antiviral activities of compounds against three RNA viruses with differing cytopathogenic properties. Dyes included alamarBlue measured by absorbance (ALB-A) and fluorescence (ALB-F), neutral red (NR), Viral ToxGlo™ (VTG), and WST-1. Viruses were chikungunya, dengue type 2, and Junin, which generally cause 100, 80-90, and 50% maximal cytopathic effect (CPE), respectively, in Vero or Vero 76 cells Compounds evaluated were 6-azauridine, BCX-4430, 3-deazaguanine, EICAR, favipiravir, infergen, mycophenolic acid (MPA), ribavirin, and tiazofurin.
View Article and Find Full Text PDFEvaluation of transmission risks associated with in vivo replication of several high containment pathogens in a biosafety level 4 laboratory.
Sci Rep
July 2014
1] Special Pathogens Program, Public Health Agency of Canada, 1015 Arlington St., Winnipeg. Manitoba [2] Departments of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada [3] Departments of Immunology, University of Manitoba, Winnipeg, MB, Canada [4] Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
Containment level 4 (CL4) laboratories studying biosafety level 4 viruses are under strict regulations to conduct nonhuman primate (NHP) studies in compliance of both animal welfare and biosafety requirements. NHPs housed in open-barred cages raise concerns about cross-contamination between animals, and accidental exposure of personnel to infectious materials. To address these concerns, two NHP experiments were performed.
View Article and Find Full Text PDFReactive astrogliosis in response to hemorrhagic fever virus: microarray profile of Junin virus-infected human astrocytes.
Virol J
July 2014
Department of Pathology, Galveston National Laboratory, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX, USA.
Background: Arenavirus Junin is the causative agent of Argentine hemorrhagic fever. Limited information is available concerning the pathogenesis of this human disease, especially the pathogenesis of acute and late neurological symptoms.
Methods: In our study we present for the first time cDNA microarray profile of human astrocytes infected with the virulent strain of Junin virus.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!