AI Article Synopsis
- The study focuses on two beneficial bacteria, Bacillus subtilis HS3 and Bacillus mycoides EC18, found in the plant root environment, examining their roles in promoting plant growth using the CRISPR-Cas9 system.
- Results indicate that B. subtilis HS3 has antifungal properties linked to compounds like fengycin and surfactin, and it selectively colonizes grass root hairs, enhancing plant growth through the release of various volatile compounds.
- For B. mycoides EC18, specific mutants showed reduced ability to promote plant growth and root colonization, suggesting that the siderophore bacillibactin is crucial for these processes and highlighting the potential of the CRISPR-Cas9 tool for studying plant
Article Abstract
Bacillus subtilis HS3 and Bacillus mycoides EC18 are two rhizosphere-associated bacteria with plant growth-promoting activity. The CRISPR-Cas9 system was implemented to study various aspects of plant-microbe interaction mechanisms of these two environmental isolates. The results show that fengycin and surfactin are involved in the antifungal activity of B. subtilis HS3. Moreover, this strain emits several other volatile organic compounds than 2,3-butanediol, contributing to plant growth promotion. Confocal laser scanning microscopy observations of the GFP-labelled strain showed that HS3 selectively colonizes root hairs of grass (Lolium perenne) in a hydroponic system. For B. mycoides EC18, we found that the wild-type EC18 strain and a ΔasbA (petropectin-deficient) mutant, but not the ΔdhbB (bacillibactin-deficient) and ADKO (asbA and dhbB double knockout) mutants, can increase the plant biomass and total chlorophyll. All the mutant strains have a reduced colonization capability on Chinese cabbage (Brassica rapa) roots, at the root tip and root hair region compared with the wild-type strain. These results indicate that the siderophore, bacillibactin, is involved in the plant growth promoting activity and could affect the root colonization of B. mycoides. Collectively, the CRISPR-Cas9 system we developed for environmental isolates is broadly applicable and will facilitate deciphering the mechanisms of Bacillus-plant interactions. © 2018 The Authors.
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| http://dx.doi.org/10.1111/1462-2920.14305 | DOI Listing |
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