Within this study the mediated detoxification of spent sulphite liquor via laccase from Trametes versicolor was analysed and optimised using an in-situ NMR-spectroscopy method. The enzymatic degradation kinetic was optimised using the degradation rate of aromatic compounds as indirect parameter. Via response surface methodology the impact of the temperature, the pH and the enzyme concentration was analysed and the conditions were optimised focusing on optimal detoxification. The results of the statistical calculation revealed a valid statistical model for the optimal impact on the aromatic degradation with a temperature of 31 °C, a pH of 6 and a laccase concentrations of 179 U g dry matter of spent sulphite liquor. By using these conditions 88.73% of aromatic compounds could be degraded.
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http://dx.doi.org/10.1016/j.jbiotec.2018.07.026 | DOI Listing |
Biotechnol Biofuels Bioprod
December 2024
Research Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1A, Vienna, A-1060, Austria.
Background: Biorefineries usually focus on the production of low-value commodities, such as bioethanol, platform chemicals or single cell protein. Shifting production to bioactive compounds, such as antimicrobial peptides, could provide an opportunity to increase the economic viability of biorefineries.
Results: Recombinant production of the antimicrobial peptide pediocin PA-1 in Corynebacterium glutamicum was transferred from yeast extract-based media to minimal media based on lignocellulosic spent sulfite liquor.
Water Res
December 2024
Department of Civil and Environmental Engineering, University of North Carolina at Charlotte, Charlotte, NC, 28223, USA.
Ion exchange (IX) can effectively remove per- and poly-fluoroalkyl substances (PFAS) from drinking water sources at ng/L to µg/L levels. However, adsorbed PFAS on spent resins should be further destructed for detoxification. Traditional resin incineration or landfilling may cause secondary pollution to the surrounding environment and cannot achieve resin reuse.
View Article and Find Full Text PDFMaterials (Basel)
August 2024
Faculty of Materials and Environmental Engineering, Technical University of Cluj-Napoca, 400641 Cluj-Napoca, Romania.
In this study, xCaO‧5FeO‧(95-x)Pb glasses and vitroceramics containing various concentrations of calcium ions (from 0 to 50 mol% CaO) were prepared using the spent anodic plate of a car battery. X-ray diffraction analysis revealed changes in the network structure as a function of CaO content. The intensities of the IR bands due to the sulfate and sulfite units were lowered, indicating a decrease in the sulfurization degree within the lead network.
View Article and Find Full Text PDFData Brief
August 2024
Research Group Bioprocess Technology, Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Gumpendorferstraße 1A, A-1060 Vienna, Austria.
The dataset consists of FTIR spectra of ultra-filtered spent sulphite liquor (UF-SSL) from softwood pulping obtained from one paper mill biorefinery plant with the purpose of quantification of the sugar content of UF-SSL. Data collection was performed using a submerged mid-IR probe placed in a continuously stirred tank reactor and reference sugar measurements were performed using HPLC. Spectra were obtained of raw and spiked UF-SSL.
View Article and Find Full Text PDFBiofabrication
July 2024
Department of Biotechnology and Food Science, Faculty of Natural Sciences, Norwegian University of Science and Technology, Trondheim, Norway.
Riboflavin overproduction bywas achieved by screening synthetic operons, enabling fine-tuned expression of the riboflavin biosynthetic genesThe synthetic operons were designed by means of predicted translational initiation rates of each open reading frame, with the best-performing selection enabling riboflavin overproduction without negatively affecting cell growth. Overexpression of the fructose-1,6-bisphosphatase () and 5-phosphoribosyl 1-pyrophosphate aminotransferase () encoding genes was then done to redirect the metabolic flux towards the riboflavin precursors. The resulting strain produced 8.
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