AI Article Synopsis
- Chromatin immunoprecipitation (ChIP) is a method used to study how proteins interact with DNA in cells.
- This technique, combined with next-generation sequencing, helps identify where specific proteins bind across the entire genome.
- The article details a protocol for isolating DNA from Vibrio cholerae that is bound to the H-NS protein, which is tagged for easy detection, and confirms that the DNA quality is suitable for sequencing.
Article Abstract
Chromatin immunoprecipitation (ChIP) measures the physical association between a protein and DNA in the cell. In combination with next-generation sequencing, the technique enables the identification of DNA targets for the corresponding protein across an entire genome. Here we describe the immunoprecipitation of Vibrio cholerae DNA bound to the histone-like nucleoid structuring protein (H-NS) tagged with the Flag epitope. The quality of the DNA obtained in this protocol is suitable for next-generation sequencing. The procedure described herein can be readily adapted to other bacteria and DNA-binding proteins.
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| http://dx.doi.org/10.1007/978-1-4939-8685-9_7 | DOI Listing |
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