This study explored the sources of nitrate and the impact of landscape pattern on nitrogen pollution in the highly urbanized Beiyun River Watershed, China during 2016 by applying a dual stable isotope approach (δN-NOand δO-NO) combined with multiple statistical analyses. The sources of riverine nitrate principally originated from manure and sewage, nitrification of soil nitrogen, fertilizer nitrification, and atmospheric deposition. A Bayesian model was used to estimate the source contributions and results showed that manure and sewage were the major contributors to river nitrate with combined proportions of 77.59% and 89.57% in the rainy season and the dry season, respectively. Results from multiple stepwise regression indicated that the typical artificial land use types and landscape configuration metrics reflecting landscape fragmentation related well with riverine nitrogen variables for different seasons (R>0.6). Industrial land, unused land and patch density of built-up land and road were positively correlated with riverine nitrogen over seasons, whereas the interspersion and juxtaposition index of forest land was negatively related with nitrate. Regulating built-up land and unused land, connecting forest land with other land use types and diminishing discharges of industrial and domestic wastewater would be effective ways to improve urban river water quality.
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