The mechanism by which microRNAs (miRNAs) modulate innate immunity and autophagy has not been fully elucidated in () infections. In this study, we identified that miR-199a inhibited key innate immune responses and autophagy in murine macrophages infected with . Using and approaches we show that the expression of miR-199a was significantly increased during infection. Furthermore, miR-199a suppressed autophagy and interferon-β (IFN-β) production by directly targeting TANK-binding kinase 1 (TBK1) mRNA in both J774a.1 and BMDM cells. Upregulation of miR-199a or TBK1 silencing (siTBK1) inhibited maturation of autophagosomes and increased survival. Our results demonstrate that, by targeting of TBK1, miR-199a modulates innate immune responses and promote the intracellular survival and growth of .
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| http://dx.doi.org/10.3389/fcimb.2018.00238 | DOI Listing |
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