AI Article Synopsis
- The study aimed to assess the impact of different intratracheal flow rates on the extracellular matrix and lung mechanics during lung decellularization.
- Healthy mice were used, with various flow rates (5, 10, and 20 ml/min) being administered through a tracheal cannula.
- Findings revealed that a flow rate of 20 ml/min resulted in better collagen preservation in decellularized lungs compared to lower rates, and this rate also showed reduced overall resistance in lung mechanics.
Article Abstract
Aim: To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
Materials & Methods: Healthy mice were used: 15 for decellularization and five to serve as controls. Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
Results: The 20 ml/min rate better preserved collagen content in decellularized lungs. Elastic fiber content decreased at 5 and 10 ml/min, but not at 20 ml/min, compared with controls. Chondroitin, heparan and dermatan content was reduced after decellularization.
Conclusion: An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
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| http://dx.doi.org/10.2217/rme-2018-0008 | DOI Listing |
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