Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.

Regulated exocytosis, which underlies many intercellular signaling events, is a tightly controlled process often triggered by calcium ion(s) (Ca). Despite considerable insight into the central components involved, namely, the core fusion machinery [soluble -ethylmaleimide-sensitive factor attachment protein receptor (SNARE)] and the principal Ca sensor [C2-domain proteins like synaptotagmin (Syt)], the molecular mechanism of Ca-dependent release has been unclear. Here, we report that the Ca-sensitive oligomers of Syt1, a conserved structural feature among several C2-domain proteins, play a critical role in orchestrating Ca-coupled vesicular release. This follows from pHluorin-based imaging of single-vesicle exocytosis in pheochromocytoma (PC12) cells showing that selective disruption of Syt1 oligomerization using a structure-directed mutation (F349A) dramatically increases the normally low levels of constitutive exocytosis to effectively occlude Ca-stimulated release. We propose a parsimonious model whereby Ca-sensitive oligomers of Syt (or a similar C2-domain protein) assembled at the site of docking physically block spontaneous fusion until disrupted by Ca Our data further suggest Ca-coupled vesicular release is triggered by removal of the inhibition, rather than by direct activation of the fusion machinery.