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Bee honey possess various nutritional and medicinal functions, which are the result of its diverse chemical composition. The numerous bioactive compounds in honey come from flower nectar; thus, the identification of the specific chemical profiles of honey samples is of great importance. The lipophilic compounds from eight monofloral honeys (rape, buckwheat, clover, willow, milk thistle, dandelion, raspberry and sweet yellow clover) were investigated. Analyses of the lipophilic fractions obtained by UAE (ultrasound assisted extraction) and SPE (solid phase extraction) extractions were performed using high-performance thin layer chromatography (HPTLC). Chromatographic and cluster analyses allowed the identification of a unique, colorful pattern of separated compounds with specific Rf values on the HPTLC plate for each type of monofloral honey. HPTLC is a simple and effective visual method of analysis, and it can serve as a basis for authenticating different types of honey.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099833 | PMC |
http://dx.doi.org/10.3390/molecules23071811 | DOI Listing |
J Med Chem
December 2024
Oncology Medicinal Chemistry Worldwide Research and Development, Pfizer, Inc., 10770 Science Center Drive, La Jolla, California 92121, United States.
Hematopoietic progenitor kinase 1 (HPK1/MAP4K1) represents a high interest target for the treatment of cancer through an immune-mediated mechanism. Herein we present highlights of the drug discovery campaign within the lactam/azalactam series of inhibitors that yielded a small molecule (, PF-07265028), which was advanced to a phase 1 clinical trial (NCT05233436). Key components of the discovery effort included optimization of potency through mitigation of ligand strain as guided by the use of cocrystal structures, mitigation of ADME liabilities (plasma instability and fraction metabolism by CYP2D6), and optimization of kinase selectivity, particularly over immune-modulating kinases with high homology to HPK1.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Tianjin Key Laboratory of Intelligent Breeding of Major Crops, Tianjin 300392, China; Tianjin Agricultural University, Tianjin 300392, China. Electronic address:
Sanxan has important application value in the field of bioactive substance delivery. However, the current sanxan emulsion gel method has certain limitations in delivering heat-sensitive lipophilic bioactive substances. In this study, the acid-gelling properties of sanxan were utilized to prepare sanxan emulsion gel microcapsules (SEGMs) by extrusion dripping method.
View Article and Find Full Text PDFAnal Chim Acta
January 2025
Department of Toxicology and Health Inspection and Quarantine, School of Public Health, Tianjin Medical University, Tianjin, 300070, China; Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin, 300070, China. Electronic address:
Background: In recent years, the increasing accumulation of estrogen pollutants in the environment has raised concerns about their impact on human health, necessitating the development of highly sensitive detection methods for trace pollutant enrichment and analysis in environmental samples. Online pretreatment detection technology offers significant advantages over conventional offline techniques, including high automation, minimal human intervention, and improved efficiency. However, the key to successful implementation lies in the advancement of novel adsorbent materials.
View Article and Find Full Text PDFAnal Chim Acta
January 2025
HAB Monitoring & Reference Branch, Stressor Detection and Impacts Division, National Centers for Coastal Ocean Science, NOAA National Ocean Service, 331 Fort Johnson Road, Charleston, SC, 29412, USA.
Background: Microcystins (MCs) and nodularins (NODs) produced by cyanobacteria occur in ambient freshwaters and across the freshwater-marine continuum, and pose health threats through drinking and recreational waters, as well as food resources. Approximately 300 MC and NOD toxins have been published, but less than 15 of them are commercially available as toxin standards. Our aim herein was to rapidly identify and quantify all toxin congeners, including those without standards, in water samples even at low abundance by reversed-phase solid phase extraction (SPE)-liquid chromatography-tandem mass spectrometry (LC-MS/MS) to provide insights into toxin levels and potential toxicity.
View Article and Find Full Text PDFTher Drug Monit
November 2024
Department of Clinical Pharmacology, The Basil Hetzel Institute for Translational Health Research, The Queen Elizabeth Hospital, Woodville South, SA, Australia; and.
Background: Immunosuppressant (IS) therapeutic drug monitoring (TDM) relies on measuring mostly pharmacologically inactive erythrocyte-bound and/or plasma protein-bound drug levels. Variations in hematocrit and plasma protein levels complicate interpretation of blood calcineurin inhibitor (CNI) and inhibitors of the molecular target of rapamycin (mTORi) concentrations. Variable binding of mycophenolic acid (MPA) to albumin similarly complicates its TDM in plasma.
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