Complete genome sequence of KLDS6.0933, a potential probiotic strain with high cholesterol removal ability.

Background: are commensal bacteria in the mammalian gastrointestinal tract which play an important role in the production of various fermented foods. Thus, certain enterococcal strains are commonly used as probiotics to confer health benefits to human and animals. KLDS6.0933 is a potential probiotic strain with high cholesterol removal ability, which was isolated from traditional naturally fermented cream in Inner Mongolia of China. To better understand the genetic basis of the probiotic properties of this strain, the whole-genome sequence was performed using the PacBio RSII platform.

Results: KLDS6.0933 contains a circular chromosome of 2,867,028 bp, two plasmids of 163,286 bp and 41,490 bp, respectively. Within the 2704 predicted genes, genes involved with acid, bile and oxidative stress resistance were identified. Bile salt hydrolase (BSH, LIANG_RS13510), a cholesterol removal enzyme identified in the KLDS6.0933 genome is different from that of other strains. Furthermore, unlike other strains, KLDS 6.0933 can facilitate the complete biosynthesis pathway of l-tryptophan.

Conclusions: In silico analysis confirmed the probiotic properties of KLDS6.0933 and may help us exploit the potential applications of KLDS6.0933 as an industrially important strain.