We have characterized the virome of a grapevine Pinot Noir accession (P70) that displayed, over the year, very stable and strong leafroll symptoms. For this, we have used two extraction methods (dsRNA and total RNA) coupled with the high throughput sequencing (HTS) Illumina technique. While a great disparity in viral sequences were observed, both approaches gave similar results, revealing a very complex infection status. Five virus and viroid isolates [Grapevine leafroll-associated viruse-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine rupestris stem pitting-associated virus (GRSPaV), Hop stunt viroid (HSVd) and Grapevine yellow speckle viroid 1 (GYSVd1)] were detected in P70 with a grand total of eleven variants being identified and de novo assembled. A comparison between both extraction methods regarding their power to detect viruses and the ease of genome assembly is also provided.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00705-018-3949-9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Inter-regional characterisation of New Zealand pinot noir wines: Correlation between wine colour, monomeric and polymeric phenolics, tannin composition, antioxidant capacity, and sensory attributes.
Food Chem
March 2025
Wine Science Programme, School of Chemical Sciences, The University of Auckland | Waipapa Taumata Rau, 23 Symonds Street, Auckland 1010, New Zealand. Electronic address:
A total of 116 New Zealand Pinot Noir wines from Central Otago (CO), Marlborough (MLB), and Martinborough (MTB) were analysed for colour, monomeric and total phenolics, antioxidant capacity, and tannins using colourimetric and HPLC methods. Correlations among chemical compositions and analytical techniques were examined. Additionally, a sensory study assessed wine colour and five mouthfeel attributes.
View Article and Find Full Text PDFGenome-wide identification of Aux/IAA gene family members in grape and functional analysis of VaIAA3 in response to cold stress.
Plant Cell Rep
October 2024
College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, China.
Article Synopsis
- Researchers identified 25 VvIAA genes from Pinot Noir and 18 VaIAA genes from Shanputao, focusing on their role in cold resistance in grapes.
- Overexpression of the VaIAA3 gene in transgenic Arabidopsis improved cold tolerance by enhancing signaling pathways related to auxin and other hormones.
- The study highlights that while the functions of IAA genes aren't fully understood in grapes, VaIAA3 shows promise for improving cold resistance, offering potential for future grape cultivation strategies.
A Machine Learning Pipeline for Predicting Pinot Noir Wine Quality from Viticulture Data: Development and Implementation.
Foods
September 2024
Centre for Advanced Computational Solutions (C-fACS), Lincoln University, Lincoln 7647, New Zealand.
The quality of wine depends upon the quality of the grapes, which, in turn, are affected by different viticulture aspects and the climate during the grape-growing season. Obtaining wine professionals' judgments of the intrinsic qualities of selected wine products is a time-consuming task. It is also expensive.
View Article and Find Full Text PDFStrategies to Increase the Phosphorus Content in the Soil Profile of Vineyards Grown in Subtropical Climates.
Plants (Basel)
August 2024
Department of Soils, Federal University of Santa Maria (UFSM), 1000 Roraima Avenue, Santa Maria 97105-900, RS, Brazil.
Phosphate fertilizers are applied to the soil surface, especially in vineyards in production in subtropical regions. Nowadays, phosphorus (P) is not incorporated into the soil to avoid mechanical damage to the root system in orchards. However, over the years, successive surface P applications can increase the P content only in the topsoil, maintaining low P levels in the subsurface, which can reduce its use by grapevines.
View Article and Find Full Text PDFStructural and Phylogenetic In Silico Characterization of PRR Protein as Potential Target for Infection.
Int J Mol Sci
September 2024
Innovative Seed Lab (ISL), Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, 28670 Villaviciosa de Odón, Spain.
Fungi infection, especially derived from , causes severe grapevine economic losses worldwide. Despite the availability of chemical treatments, looking for eco-friendly ways to control infection is gaining much more attention. When a plant is infected, multiple disease-control molecular mechanisms are activated.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!