AI Article Synopsis
- - The study assessed three different DNA extraction methods to confirm the presence of Mycobacterium avium subspecies paratuberculosis in bovine feces samples.
- - The DNA Extract All Reagents Kit™ showed the highest efficiency, successfully extracting amplifiable DNA from 96.29% of known positive samples.
- - In contrast, the other methods, Chelex-100 resin and polyethylene glycol, had significantly lower success rates at 25.92% and 0%, respectively.
Article Abstract
We evaluated three DNA extraction methods for confirmation of Mycobacterium avium subspecies paratuberculosis from liquid cultures of bovine feces. Use of DNA Extract All Reagents Kit™ resulted in efficient extraction of amplifiable DNA from higher proportion (96.29%) of known positive samples compared to Chelex-100 resin (25.92%) and polyethylene glycol (0%).
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| http://dx.doi.org/10.1016/j.mimet.2018.07.012 | DOI Listing |
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