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Effects of five tissue sources of silver carp by-products on the structure, physicochemical and emulsifying properties of gelatin.
Curr Res Food Sci
October 2024
Medical Food Laboratory, Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai Institute for Pediatric Research, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.
Article Synopsis
- The study explored how different body parts of silver carp (skin, scale, fin, head, and bone) affect the properties of gelatins extracted from them.
- Results showed that skin and scale gelatins have better water-holding, fat-binding capacities, and overall gel strength compared to gelatins from fins and bones.
- The findings are significant for understanding how these tissue sources can influence gelatin's use in food products, as gelatins vary in their ability to stabilize emulsions and their physical characteristics.
The Regulatory Role of miRNAs in Zebrafish Fin Regeneration.
Int J Mol Sci
September 2024
Molecular and Genetic Laboratory, College of Life Science, Henan Normal University, 46# East of Construction Road, Xinxiang 453007, China.
Article Synopsis
- - Research on zebrafish fins, which possess strong regenerative abilities, reveals that miRNAs (small regulatory molecules) play a significant role in controlling gene expression during fin regeneration.
- - When miRNA agomirs were injected into zebrafish, blastema cell proliferation and overall fin regeneration were notably reduced due to the suppression of specific genes and proteins linked to blastocyte formation, notably those in the Hippo signaling pathway.
- - The study also found that miRNAs negatively impact the regeneration of fin rays by inhibiting bone-related gene expression, providing insights for future research on bone reconstruction in aquatic vertebrates.
WISH-BONE: Whole-mount in situ histology, to label osteocyte mRNA and protein in 3D adult mouse bones.
FASEB J
October 2024
Department of Bioengineering, Northeastern University, Boston, Massachusetts, USA.
Article Synopsis
- Bone is a constantly changing 3D tissue, and traditional methods for studying it often require sample preparation that removes important spatial information.* -
- The WISH-BONE method presented here allows researchers to label mRNA and proteins in whole mouse bones, preserving their 3D structure for better analysis.* -
- This technique provides new protocols for understanding molecular interactions in osteocytes, offering potential applications in various areas of bone research despite some limitations in detection sensitivity.*
The Osteoblast Transcriptome in Developing Zebrafish Reveals Key Roles for Extracellular Matrix Proteins Col10a1a and Fbln1 in Skeletal Development and Homeostasis.
Biomolecules
January 2024
Laboratory for Organogenesis and Regeneration (LOR), GIGA Institute, University of Liège, 4000 Liège, Belgium.
Article Synopsis
- Zebrafish are being extensively used to study bone development and diseases, emphasizing the need to understand how osteoblasts differentiate and function.
- Researchers isolated specific osteoblast subpopulations from young zebrafish to examine their unique genetic and signaling characteristics.
- Mutations in genes related to extracellular matrix proteins showcased significant alterations in skeletal structure and mineral density in zebrafish, underlining the proteins' crucial role in bone development and maintenance.
A comprehensive coding and microRNA transcriptome of vertebral bone in postlarvae and juveniles of Senegalese sole (Solea senegalensis).
Genomics
March 2024
Department of Anatomy, Animal Production and Veterinary Clinical Sciences, Faculty of Veterinary, Campus Terra, Universidade de Santiago de Compostela, 27002 Lugo, Spain.
Understanding vertebral bone development is essential to prevent skeletal malformations in farmed fish related to genetic and environmental factors. This is an important issue in Solea senegalensis, with special impact of spinal anomalies in postlarval and juvenile stages. Vertebral bone transcriptomics in farmed fish mainly comes from coding genes, and barely on miRNA expression.
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