AI Article Synopsis
- The study focuses on the roles of two key costimulatory molecules, CD40 and CD154, in immune responses in grass carp, particularly in their ability to produce antibodies against infections.
- Researchers identified and analyzed the cDNAs of CD40 and CD154, finding that their expression levels increase in immune organs after exposure to grass carp reovirus (GCRV), specifically peaking at 72 hours post-infection.
- The study also discovered several single nucleotide polymorphisms (SNPs) in the CD40 gene that are significantly associated with grass carp's resistance to GCRV, suggesting that these SNP markers could aid in breeding disease-resistant fish.
Article Abstract
In bony fish, CD40 and CD154 are two very important costimulatory molecules involved in T and B cell cooperation in thymus-dependent antibody production. In the current study, we identified the cDNAs of CD40 and CD154 and analyzed their genomic structures in grass carp. Quantitative real-time PCR indicated that the CD40 and CD154 were mainly expressed in immune organs. After challenge with grass carp reovirus (GCRV), these two genes were up-regulated at 72 h in head kidney and spleen. Moreover, seven and five single nucleotide polymorphisms (SNPs) were identified in the CD40 and CD154 respectively. Statistical analysis indicated that three SNPs in the coding region of the CD40 were significantly associated with the resistance of grass carp against GCRV. These results indicated that CD40 and CD154 play important roles in the responses to GCRV in grass carp. The SNP markers in the CD40 associated with the resistance to GCRV may facilitate the disease-resistant breeding of grass carp.
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| http://dx.doi.org/10.1016/j.fsi.2018.07.037 | DOI Listing |
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