AI Article Synopsis
- The study identifies two multi-drug resistant strains of E. coli from a hospital in Milan, linked to surgical and surveillance samples, which tested positive for the blaNDM-type gene.
- Whole-genome sequencing revealed multiple resistance genes, including blaNDM-5, blaCMY-42, and others, indicating significant antibiotic resistance.
- Both strains belong to phylogenetic group A, sequence type ST1702, and possess an IncI1 plasmid for gene localization, marking the first occurrence of this specific E. coli clone in Italy.
Article Abstract
We describe two multi drug-resistant (MDR) carbapenemase-producing Escherichia coli clinical isolates from an acute hospital in Milan. Both strains, isolated from a surgical wound sample and a surveillance rectal swab respectively, were positive for a blaNDM-type gene by Xpert Carba-R test. The whole-genome sequence characterization disclosed several resistance determinants: blaNDM-5, blaCMY-42, blaTEM-198, rmtB, mphA. The two isolates belonged to phylogenetic group A, sequence type (ST) 1702 and serotype O89:H9. PCR-based replicon typing and conjugation assay demonstrated an IncI1 plasmid localization for both blaNDM-5 and blaCMY-42 genes. This is the first report of a ST1702 NDM-5 and CMY-42- producing E. coli clone in Italy.
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