AI Article Synopsis
- - The study developed a budget-friendly method for producing bioethanol using a specific yeast strain (S. cerevisiae F118) and concentrated sweet sorghum juice, achieving a higher ethanol yield than a standard strain.
- - The F118 strain achieved an impressive ethanol titer of 86.19 g/L, outperforming the non-flocculent BY4741 strain, which produced only 33.92 g/L, due to better gene expression linked to sugar processing.
- - After multiple fermentation cycles, the F118 strain demonstrated successful separation from the liquid through floc formation, resulting in an ethanol concentration of 100.37 g/L by the fifth cycle.
Article Abstract
The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L.
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| http://dx.doi.org/10.1016/j.biortech.2018.07.039 | DOI Listing |
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