TbRRM1, an SR-related protein, is involved in transcriptional and post-transcriptional gene expression regulation in procyclic T. brucei. In previous work, we found that TbRRM1 is essential and its depletion leads to cell cycle impairment, aberrant phenotypes and cell loss by apoptotic-like death. Here, we report the findings obtained after TbRRM1 knockdown in bloodstream parasites. Depletion of TbRRM1 in this cell stage led also to growth arrest and cell loss by apoptosis-like death. However, microscopic analysis showed aberrant cell morphology with parasites displaying flagellum detachment and cytokinesis impairment after RNAi induction, suggesting that TbRRM1 could play different roles depending on parasite stage.
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| http://dx.doi.org/10.1016/j.molbiopara.2018.07.006 | DOI Listing |
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The Trypanosoma brucei RNA-Binding Protein TbRRM1 is Involved in the Transcription of a Subset of RNA Pol II-Dependent Genes.
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Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (IIB-UNSAM) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 25 de Mayo y Francia, San Martín, Buenos Aires, Argentina.
It has been long thought that RNA Polymerase (Pol) II transcriptional regulation does not operate in trypanosomes. However, recent reports have suggested that these organisms could regulate RNA Pol II transcription by epigenetic mechanisms. In this paper, we investigated the role of TbRRM1 in transcriptional regulation of RNA Pol II-dependent genes by focusing both in genes located in a particular polycistronic transcription unit (PTU) and in the monocistronic units of the SL-RNA genes.
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Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (IIB-UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 25 de Mayo y Francia, Gral. San Martín, 1650 Buenos Aires, Argentina. Electronic address:
TbRRM1, an SR-related protein, is involved in transcriptional and post-transcriptional gene expression regulation in procyclic T. brucei. In previous work, we found that TbRRM1 is essential and its depletion leads to cell cycle impairment, aberrant phenotypes and cell loss by apoptotic-like death.
View Article and Find Full Text PDFTrypanosoma brucei RRM1 is a nuclear RNA-binding protein and modulator of chromatin structure.
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March 2015
Institute of Cell Biology, University of Bern, Bern, Switzerland
Unlabelled: TbRRM1 of Trypanosoma brucei is a nucleoprotein that was previously identified in a search for splicing factors in T. brucei. We show that TbRRM1 associates with mRNAs and with the auxiliary splicing factor polypyrimidine tract-binding protein 2, but not with components of the core spliceosome.
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