β-D-glucosidase (βG) gene from Oenococcus oeni SD-2a and 31MBR was cloned, sequenced and analyzed, also intracellular βG of the two strains was further localized. The results showed that βG gene of the two strains was in high homology (> 99%) to reported βG gene, con-firming both strains possess βG activity at the molecular level. Intracellular βG of SD-2a is a mainly soluble protein, existing mostly in the cytoplasm and to some extent in the periplasm. While for 31MBR, intracellular βG is mainly insoluble protein existing in the cytoplasmic membrane. This study provides basic information for further study of the metabolic mechanism of βG from O. oeni SD-2a and 31MBR.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.5604/17331331.1204481 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploration of N6-methyladenosine modification in ascorbic acid 2-glucoside constructed stem cell sheets.
J Mol Histol
October 2024
Department of Neonatal Intensive Care Unit, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, No. 106 of Zhongshan Er Road, Yuexiu District, Guangzhou, 510080, China.
The aim of this study was to explore the mechanism of bone marrow stem cells (BMSCs) sheets constructed with different doses of Ascorbic acid 2-glucoside (AA-2G) in conjunction with N6-methyladenosine (m6A)-associated epigenetic genes analysing transcriptome sequencing data. Experimental groups of BMSCs induced by different AA-2G concentrations were set up, and the tissue structures were observed by histological staining of cell slices and scanning electron microscopy. Expression patterns of DEGs were analysed using short-time sequence expression mining software, and DEGs associated with m6A were selected for gene ontology analysis and pathway analysis.
View Article and Find Full Text PDFPromoter engineering for efficient production of sucrose phosphorylase in Bacillus subtilis and its application in enzymatic synthesis of 2-O-α-D-glucopyranosyl-L-ascorbic acid.
Enzyme Microb Technol
September 2023
Institute of Fermentation Engineering, Zhejiang University of Technology, Hangzhou 310014, China; College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China. Electronic address:
2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G), a stable glucoside derivative of L-ascorbic acid (L-AA), can be one-step synthesized by sucrose phosphorylase (SPase). In this study, we attempted to produce extracellular SPase in Bacillus subtilis WB800 for the food-grade production of AA-2G. The results showed that the secretion of SPases did not require signal peptide.
View Article and Find Full Text PDFActivating transcription factor-2 supports the antioxidant capacity and ability of human mesenchymal stem cells to prevent asthmatic airway inflammation.
Exp Mol Med
February 2023
Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, South Korea.
Article Synopsis
- Glutathione (GSH) is a helpful molecule that helps stem cells stay healthy and work properly, but scientists don’t fully understand how it works yet.
- A protein called ATF2 is important for keeping GSH at the right levels in stem cells by working with another protein called NRF2.
- By using a special form of vitamin C, researchers found that activating ATF2 can improve how well stem cells work, which is important for treating conditions like asthma.
[Comparison of three α-glucosidases from different sources in the synthesis of -ascorbic acid 2-glucoside].
Sheng Wu Gong Cheng Xue Bao
July 2022
Institute of Biomedicine, Jinan University, Guangzhou 510632, Guangdong, China.
-ascorbic acid 2-glucoside (AA-2G) is a derivative of -ascorbic acid (-AA). Compared with -AA, it has good stability and is easily decomposed by enzyme in the human body. α-Glucosidase (AG) was the first enzyme found capable of producing AA-2G.
View Article and Find Full Text PDFHeterologous Expression of Cyclodextrin Glycosyltransferase 20 in and Its Application in 2--α-D-Glucopyranosyl-L-Ascorbic Acid Production.
Front Microbiol
May 2021
Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
In this study, the cgt gene 20, which encodes cyclodextrin glycosyltransferase (CGTase) and was obtained by the metagenome sequencing of marine microorganisms from the Mariana Trench, was codon optimized and connected to pET-24a for heterologous expression in BL21(DE3). Through shaking flask fermentation, the optimized condition for recombinant CGTase expression was identified as 20°C for 18 h with 0.4 mM of isopropyl β-D-L-thiogalactopyranoside.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!