[Study on the Relationship Between Gene and Resistance of Taxol in Ovarian Cancer].

Objective: To research the expression of X-linked inhibitor of apoptosis protein gene () on paclitaxel resistance in ovarian cancer.

Methods: A2780 and A2780/T cells were treated with paclitaxel respectively at the concentrations of 5 ng/mL,10 ng/mL,20 ng/mL , 40 ng/mL,80 ng/mL,160 ng/mL,320 ng/mL,then the inhibition rate of cells were detected by MTT assay. The expression of mRNA and protein among the A2780 and A2780/T cells treated respectively with paclitaxel at the concentration of 100 ng/mL was detected by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot. The A2780/T cells were divided into blank group,empty group,small interfering RNA (siRNA) group and siRNA-non-specific group. The expression of mRNA and protein of four groups were detected by RT-qPCR and Western blot. Apoptotic rate of these groups with addition of paclitaxel at the concentrations of 0 ng/mL,1 000 ng/mL,1 500 ng/mL,2 000 ng/mL and 2 500 ng/mL were detected by flow cytometry.

Results: After the treatments on A2780 and A2780/T cells with the different concentrations of paclitaxel,the inhibition rate of A2780 cells were gradually increased with the increased paclitaxel concentrations (<0.05),while there were no obvious differences in A2780/T cells (>0.05). After the treatment on these cells with paclitaxel at the concentration of 100 ng/mL,the expression of mRNA was lower than that non-treatment with paclitaxelin A2780 cells (<0.05),and the expression of mRNA in the A2780/T cells were no statistical significance between the treatment group and non-treatment group with paclitaxel (>0.05). However,the expression of A2780/T cells' mRNA and protein treated with paclitaxel were higher than A2780 cells' (<0.05). The expression of mRNA and protein in siRNA- group was lower than those of other groups (<0.05). The apoptotic rate of siRNA- group was higher than those of other groups treated with the paclitaxel at concentrations of 2 000 ng/mL and 2 500 ng/mL (<0.05).

Conclusion: 's high expression on mRNA and protein was correlated with ovarian cancer paclitaxel-resistance,specific siRNA can promote cell apoptosis by reducing the expression of ,and increase the sensitivity of drug-resistant cancer cells to paclitaxel.