Overexpression of inhibits proliferation and promotes apoptosis of human laryngeal squamous carcinoma cell Hep-2.

Objective: was previously found to be downregulated in human laryngeal cancer (LC) tissues, and it results in poor prognosis. This study aimed to further confirm the antitumor effects of in LC cell line Hep-2.

Materials And Methods: Beclin 1 was overexpressed in Hep-2 cells using liposomal transfection and confirmed using reverse transcription polymerase chain reaction and Western blotting. Then, cell proliferation and apoptosis were determined in control (untransfected), empty vector transfected, and overexpressed groups using MTT and flow cytometry procedure, respectively.

Results: The expression of the gene in Hep-2 cells was significantly increased after vector transfection compared with control (1.173±0.046 vs 0.453±0.016, <0.01) and empty vector (1.173±0.046 vs 0.440±0.021, <0.01). Overexpression of inhibited proliferation at 4 days (0.619±0.051 vs 0.891±0.081 and 0.619±0.051 vs 0.878±0.105, <0.01), 5 days (0.684±0.078 vs 1.127±0.094 and 0.684±0.078 vs 1.162±0.117, <0.01), and 6 days (0.725±0.069 vs 1.168±0.103 and 0.725±0.069 vs 1.194±0.097, <0.01) and promoted apoptosis (14.48%±1.42% vs 4.07%±0.66% and 14.48%±1.42% vs 4.39%±0.80%, <0.01) in Hep-2 cells in comparison with the control and empty vector groups, respectively.

Conclusion: may be an underlying target for the treatment of LC. This study has provided some experimental basis for the gene therapy of LC.