Background: Osteosarcoma (OS) is a common malignant tumor that predominantly occurs in adolescents. Its most common metastasis is to the lungs. As shown in our earlier study, lysosome-associated membrane glycoprotein 3 (LAMP3) is highly upregulated in metastatic OS. However, its role in the regulation of OS cell viability and apoptosis remains unknown.
Methods: We knocked down and overexpressed in OS cells and assessed the cell viability and apoptosis. Then, we investigated the expression of apoptosis-associated genes to identify the downstream gene(s) of .
Results: Knockdown of significantly inhibited OS cell viability and promoted apoptosis. , which is involved in the apoptosis pathway, was found to be highly upregulated after knockdown of . Overexpression of significantly increased cell viability and abrogated apoptosis. Importantly, subsequent knockdown of partially suppressed the increased OS cell apoptosis induced by the inhibition of , suggesting that is a key functional downstream gene of .
Conclusions: Our findings suggest that promotes OS cell viability and survival by regulating expression.
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| http://dx.doi.org/10.1186/s11658-018-0099-8 | DOI Listing |
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