Magnetic extraction and purification of DNA from whole human blood using a γ-FeO@Chitosan@Polyaniline hybrid nanocomposite.

Carbohydr Polym

Pós-Graduação em Ciência de Materiais, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil; Departamento de Física, Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil. Electronic address:

Published: October 2018

AI Article Synopsis

  • The study evaluated the nanocomposite γ-FeO@Chi@Pani for its ability to adsorb DNA from solutions, particularly salmon sperm DNA, using UV-vis absorption techniques.
  • The nanocomposite demonstrated an adsorption capacity of 49.5 mg/g, and the DNA capture process involved both chemical and diffusion mechanisms.
  • When tested with human blood samples, it successfully extracted DNA at a rate of 80 ng/μL, indicating its potential for effective DNA purification in complex biological samples.

Article Abstract

We examined the properties of the nanocomposite γ-FeO@Chi@Pani as an adsorbent of deoxyribonucleic acid (DNA). As a model system, we used an aqueous solution of salmon sperm DNA, whose decreasing concentration was followed by monitoring the 260 nm UV-vis absorption. After adjusting the data collected to a Langmuir isotherm curve, we estimated the adsorption capacity (q) of the nanocomposite as 49.5 mg/g. We also observed that the kinetic model of the DNA capture presents a mixed character, with both chemical mechanisms and intraparticle diffusion processes involved. When the MNC was used to extract the DNA from complex samples (human blood), a capture rate of 80 ng/μL was achieved, with the collected fraction exhibiting good quality, as evaluated by PCR analysis and electrophoresis assays. These results suggest that the γ-FeO@Chi@Pani nanocomposite is a promising adsorbent for use in protocols for purification of DNA from complex samples.

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Source
http://dx.doi.org/10.1016/j.carbpol.2018.05.034DOI Listing

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