Background: The small bowel is prone to ischemic injury during transport before transplantation, an injury that endangers the recipient patient. The small-bowel mucosal microcirculation in particular appears to be highly sensitive to injury. Current preservation solutions such as histidine-tryptophan-ketoglutarate (HTK) solution provide some protection to the graft. However, these were developed decades ago and do not address several critical processes, such as hypoxia-induced membrane pores and free radical-mediated hypothermic injury.
Methods: To protect the graft from cold ischemic injury, we implemented a modified HTK solution here, including glycine, alanine, and iron chelators in a heterotopic, syngeneic small-bowel transplantation model of the rat. The effects of the modified solution and its major components were compared against the conventional HTK solution using intravital microscopy in the early reperfusion period.
Results: The amino acid glycine, added to HTK solution, slightly improved mucosal perfusion. Both, the modified base solution (without iron chelators) and iron chelators increased functional capillary density of the mucosa during the early reperfusion period. The complete modified solution (with glycine, alanine, and iron chelators) significantly increased the perfusion index, functional capillary density of the mucosa, and red blood cell velocity in the grafts after reperfusion in comparison with the grafts preserved with HTK.
Conclusions: The modified preservation solution improved the microcirculation of the transplants and needs detailed evaluation in further models of small-bowel transplantation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/TP.0000000000002318 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The cardioprotective effects of HTK solution and conventional blood cardioplegia in patients with atrial fibrillation undergoing valvular replacement and Cox maze III procedure.
J Cardiothorac Surg
January 2025
Department of Anesthesiology, Women's Hospital of Nanjing Medical University, Nanjing Women and Children's Healthcare Hospital, No. 123, Tianfei Lane, Mochou Road, Nanjing, Jiangsu, 210004, China.
Introduction: The study was to assess the myocardial protection effects of the histidine-tryptophan-ketoglutarate (HTK) solution and the 4:1 blood cardioplegia (BC) in patients with atrial fibrillation (AF) who were subjected to valvular replacement concomitant with the Cox maze III surgery.
Methods: A cohort of 148 individuals afflicted with AF, who received valve replacement surgery in conjunction with the Cox maze III procedure at our clinic within the period extending from 2015 to 2023, were enrolled. Subsequent to adjustment by propensity score matching (PSM), the patients were categorized into two distinct groups: the HTK group and the BC group.
A Novel Machine Perfusion System for Enhancing Hepatic Microcirculation Perfusion.
Artif Organs
December 2024
Hubei Provincial Clinical Research Center for Natural Polymer Biological Liver, Hubei Key Laboratory of Medical Technology on Transplantation, National Quality Control Center for Donated Organ Procurement, Transplant Center of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China.
Background: Machine perfusion is a promising strategy for safeguarding liver transplants donated after cardiac death (DCD). In this study, we developed and validated a novel machine perfusion approach for mitigating risk factors and salvaging severe DCD livers.
Methods: A novel hypothermic oxygenated perfusion (HOPE) system was developed, incorporating two pumps and an elastic water sac to emulate the functionality of the cardiac cycle.
Preservation solution protects isolated hair micrografts by inhibiting apoptosis of hair bulb.
Life Sci
January 2025
Department of Plastic and Aesthetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China. Electronic address:
Aims: To investigate the effectiveness of histidine-tryptophan-ketoglutarate (HTK) solution compared to Ringer's (RS) solution for preserving isolated hair follicles (HFs), focusing on structural integrity, cell viability, apoptosis prevention, and identifying the mechanisms of cell death during the preservation period.
Materials And Methods: Isolated human HFs were preserved in HTK or RS solution for periods ranging from 2 to 12 h. Morphological changes were assessed using H&E staining and transmission electron microscopy (TEM).
HTK Solution Cardioplegia in Pediatric Patients: A Meta-analysis.
Thorac Cardiovasc Surg
November 2024
Prevent Senior Private Health Operator, Sao Paulo, Brazil.
Introduction: Cardioplegia, a therapy designed to induce reversible cardiac arrest, revolutionized cardiovascular surgery. Among the various pharmacological approaches is the histidine-tryptophan-ketoglutarate (HTK) solution. Despite numerous studies, no meta-analysis has investigated the efficacy of the HTK solution in the pediatric population.
View Article and Find Full Text PDFMitochondrial respiratory pathways in immature rat heart tissue using different cardioplegic solutions.
Gen Thorac Cardiovasc Surg
November 2024
Institute of Physiology and Pharmacology, Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Article Synopsis
- Minor defects in the mitochondrial ATP-generating system can harm heart function in immature hearts, so this study explored various cardioplegic solutions.
- The experiment involved 41 male rats and compared the effects of three solutions (St. Thomas, Custodiol HTK, and Del Nido) during 1-hour and 3-hour ischemic periods.
- Results showed that the Del Nido solution offered the best mitochondrial respiration and ATP production compared to the other solutions, particularly after extended ischemia.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!