Hydrogen peroxide modulates redox status, energy metabolism, and gene expression in a dose- and time-dependent manner in rat liver.

Aim: The aim of this study was to investigate the effect of three different hydrogen peroxide (H O ) levels on blood and liver oxidative status, energy metabolites, and gene expression in male albino rats at two time intervals (2 and 4 weeks).

Methods: A total of 32 rats were divided into four groups. The first group received tap water and served as control. The second group received low dose of hydrogen peroxide (H O ; 0.25%), The third group received medium dose of H O (0.5%) and the fourth group received high dose of H O (1%) in drinking water.

Results: Present data showed that medium and high dose increased oxidative stress markers, decreased cell energy, and decreased antioxidant enzyme gene expression (GPx and Nrf2) and its downstream in contrast low dose did not show significant effects.

Conclusion: This study might indicate that hydrogen peroxide medium level is the best dose for redox model status.