Whole-genome sequencing of a CTX-M-11-encoding and quinolone-non-susceptible Klebsiella pneumoniae ST194 isolate from a hospitalised dog in Greece.

Objectives: The emergence and spread of transferable β-lactamases among Enterobacteriaceae is a major problem both to human and veterinary medicine and is an important contributing factor to the development of multidrug-resistant bacterial isolates. In the present study, whole-genome sequencing of a Klebsiella pneumoniae isolate (LKP817909) resistant to first- and second-generation cephalosporins and non-susceptible to fluoroquinolones, isolated from a urine sample of a hospitalised dog, was performed.

Methods: Genome sequencing was performed on an Illumina MiniSeq Sequencing System. Multilocus sequence typing (MLST) was performed using a BLAST-based approach, whereas antimicrobial resistance genes and plasmid replicons were identified by ResFinder and PlasmidFinder, respectively. The Rapid Annotation using Subsystem Technology (RAST) server v.2.0 was used for genome annotation.

Results: Data analyses revealed the complete resistome of isolate LKP817909, which included the cefotaximase-München-11 (CTX-M-11) extended-spectrum β-lactamase together with 11 other resistance genes. Ten resistance genes were located on plasmids and two on the chromosome.

Conclusions: To the best of our knowledge, this is the first detection of a CTX-M-11-producing K. pneumoniae isolated from a canine. The whole genome sequence of the isolate has been deposited at GenBank to serve as a future reference.