AI Article Synopsis
- Somaclonal variation in regenerated plants can lead to DNA abnormalities such as aneuploidy and polyploidy.
- Flow cytometry (FCM) utilizing propidium iodide is an effective method for assessing DNA content and ploidy variation in plant tissue cultures.
- The study outlines a two-step method for preparing samples and analyzes DNA ploidy stability in banana plants regenerated from embryogenic cell suspensions.
Article Abstract
Somaclonal variation (SC) in plants regenerated from tissue culture, via organogenesis or somatic embryogenesis, is frequently associated with abnormalities in the content of deoxyribonucleic acid (DNA), viz., aneuploidy and polyploidy. Flow cytometry (FCM) using the nucleic acid-specific fluorochrome propidium iodide has proven to be a rapid, simple, and reproducible technique for assessment of DNA content and ploidy variation occurring in plant tissue cultures. Here an outline of the sample preparation of suspension with intact nuclei by the two-step standard method, and FCM analysis of DNA ploidy stability in plants regenerated from embryogenic cell suspension (ECS) of banana Musa acuminata, AAA, cv. Grand Naine (Cavendish subgroup) using an internal standard is described.
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| http://dx.doi.org/10.1007/978-1-4939-8594-4_22 | DOI Listing |
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