Development and characterization of 22 polymorphic microsatellites of the Andean frog Telmatobius chusmisensis (Anura, Telmatobius) and cross amplification in seven Chilean species of the genus.

Mol Biol Rep

Laboratorio de Genética y Evolución, Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Santiago, Chile.

Published: October 2018

AI Article Synopsis

  • The genus Telmatobius is a diverse group of amphibians found in high-altitude Andean areas, with several species in Chile being endangered or critically endangered.
  • Researchers identified and analyzed 44 microsatellite markers from 80 individuals across 8 species, successfully obtaining 22 functional markers for Telmatobius chusmisensis.
  • These microsatellite markers are essential for future genetic studies, helping in understanding population dynamics and aiding conservation efforts for the endangered Telmatobius species.

Article Abstract

The genus Telmatobius Wiegmann, 1834 is composed of a wide variety of species and is one of the most diverse native components of the high-altitude Andean environments. The species of the genus present in Chile are considered as endangered, critically endangered or data deficient. We isolated and evaluated 44 microsatellites in 80 individuals of 8 species of Telmatobius present in Chile, obtaining 22 polymorphic microsatellite loci for Telmatobius chusmisensis. The cross-amplification test was successful in all other species tested. For the first time, microsatellite markers are described for Telmatobius. The description of these primers will be useful for further genetic studies for T. chusmisensis and other species of the same genus; allowing further analyses of population structuring, dispersal patterns, recent demographic history and population effective size. This information is also significant to undertake conservation actions for the species of the genus Telmatobius, since most species have conservation issues.

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http://dx.doi.org/10.1007/s11033-018-4228-2DOI Listing

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