Objective: To analyse the immunohistochemical expression of ameloblastin in the bell stage of tooth germ and compare with ameloblastoma to determine the level of differentiation of tumour cells.
Study Design: This study included eleven human tooth germs with four in the early and seven in the late bell stage, and six selected archival tissue samples of ameloblastomas were studied using haematoxylin and eosin, Masson's trichrome and ameloblastin.
Results: All eleven tooth germs reacted positively to ameloblastin with a characteristic inverted and sequential pattern of expression in the acellular zone of the dental papilla and enamel organ. Of the six cases of ameloblastoma, five cases showed a variable level of expression of ameloblastin in the tumour cells, whereas in one case, ameloblastin was negative in the tumour cells but positive in the stromal fibrous tissue collar.
Conclusion: Expression of ameloblastin in human tooth germ is related to differentiation and mineralization, and it correlates with the state of differentiation of the tumour cells in ameloblastoma.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/odi.12934 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
LAP-MALDI MS analysis of amelogenin from teeth for biological sex estimation.
J Pharm Biomed Anal
November 2024
Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6DX, United Kingdom. Electronic address:
The biological sex estimation of human individuals can be achieved by extracting fragments of the amelogenin protein from small areas of tooth enamel. The amelogenin gene can be found on both sex chromosomes (X and Y) with chromosome-specific differences in its sequence, and consequently the sequences of the expressed protein in teeth. Virtually all current analytical techniques used to identify the occurrence of the male Y chromosome-specific proteoform employ proteoform-specific peptide analysis by LC-ESI MS/MS, which typically results in longer analytical times due to the LC separation step, despite recent efforts of shortening the LC step.
View Article and Find Full Text PDFThe sensory nerve regulates stem cell homeostasis through Wnt5a signaling.
iScience
October 2024
Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China.
Article Synopsis
- Nerves play a crucial role in maintaining the balance and development of epithelial stem cells, particularly during tooth development in rats.
- A study showed that when sensory nerves were damaged, it led to issues like impaired mineralization and thinner enamel in the rats' incisors.
- Key enamel-related factors, such as ameloblastin and amelogenin, were found to be less expressed after nerve injury, suggesting that sensory nerves are important for proper enamel formation and mineralization.
An unexpected role of neurite outgrowth inhibitor A as regulator of tooth enamel formation.
Int J Oral Sci
October 2024
Orofacial Development and Regeneration, Institute of Oral Biology, University of Zürich, Zürich, Switzerland.
Neurite outgrowth inhibitor A (Nogo-A) is a major player in neural development and regeneration and the target of clinical trials aiming at promoting the regeneration of the central nervous system upon traumatic and ischemic injury. In this work, we investigated the functions of Nogo-A during tooth development to determine its role in dental physiology and pathology. Using immunohistochemistry and in situ hybridization techniques, we showed that Nogo-A is highly expressed in the developing mouse teeth and, most specifically, in the ameloblasts that are responsible for the formation of enamel.
View Article and Find Full Text PDFRoles of the histone methyltransferase SET domain bifurcated 1 in epithelial cells during tooth development.
Arch Oral Biol
September 2024
Maxillofacial Orthognathics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
Objective: This study aimed to reveal the effects of SET domain bifurcated 1 (SETDB1) on epithelial cells during tooth development.
Design: We generated conditional knockout mice (Setdb1 mice), in which Setdb1 was deleted only in epithelial cells. At embryonic day 14.
Regulatory role of N-myc downregulated genes in amelogenesis in rats.
J Mol Histol
April 2024
Department of Oral Anatomy, Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, 500-757, Republic of Korea.
Cytodifferentiation of odontogenic cells, a late stage event in odontogenesis is based on gene regulation. However, studies on the identification of the involved genes are scarce. The present study aimed to search for molecules for the cytodifferentiation of ameloblastic cells in rats.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!