AI Article Synopsis
Article Abstract
Ischaemia-reperfusion injury (IRI) during various surgical procedures, including partial nephrectomy for kidney cancer or renal transplantation, is a major cause of acute kidney injury and chronic kidney disease. Currently there are no drugs or methods for protecting human organs, including the kidneys, against the peril of IRI. The aim of this study was therefore to investigate the reno-protective effect of Zn preconditioning in a clinically relevant large animal sheep model of IRI. Further the reno-protective effectiveness of Zn preconditioning was tested on normal human kidney cell lines HK-2 and HEK293. Anaesthetised sheep were subjected to uninephrectomy and 60 min of renal ischaemia followed by reperfusion. Sheep were preconditioned with intravenous injection of zinc chloride prior to occlusion. Serum creatinine and urea were measured before ischaemia and for 7 days after reperfusion. HK-2 and HEK293 cells were subjected to in vitro IRI using the oxygen- and glucose-deprivation model. Zn preconditioning reduced ischaemic burden determined by creatinine and urea rise over time by ~ 70% in sheep. Zn preconditioning also increased the survival of normal human kidney cells subjected to cellular stress such as hypoxia, hydrogen peroxide injury, and serum starvation. Overall, our protocol incorporating specific Zn dosage, number of dosages (two), time of injection (24 and 4 h prior), mode of Zn delivery (IV) and testing of efficacy in a rat model, a large preclinical sheep model of IRI and cells of human origin has laid the foundation for assessment of the benefit of Zn preconditioning for human applications.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10534-018-0125-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Concomitant Robot-Assisted Partial Nephrectomy and Pyelolithotomy for Small Renal Cell Carcinoma and Partial Staghorn Calculus in the Same Kidney: A Case Report and Literature Review.
Asian J Endosc Surg
December 2024
Department of Urology, Shonan-Fujisawa Tokushukai Hospital, Fujisawa, Japan.
Management of a small renal mass and a renal stone in the same kidney presents several dilemmas. Simultaneous robot-assisted partial nephrectomy and pyelolithotomy via the same surgical access site is a reasonable approach in a patient with both entities. An 80-year-old woman was diagnosed with a 2.
View Article and Find Full Text PDFThe role of continuous renal replacement therapy in the management of cardiorenal syndrome involving acute myocardial infarction with concomitant pneumonia: case report.
Pan Afr Med J
December 2024
Department of Surgery, Faculty of Medicine, Hasanuddin University, Hasanuddin University Hospital, Makassar, Indonesia.
Acute heart failure is associated with high hospitalization and mortality rates. A strong, independent risk factor for mortality in patients with heart failure is acute kidney injury, and the condition caused by this connection between disturbances in heart function and proper kidney functioning is cardiorenal syndrome (CRS). This case report discusses the role of continuous renal replacement therapy (CRRT) in the management of a CRS case with septic shock due to pneumonia.
View Article and Find Full Text PDFUnveiling the intricate interplay: Exploring biological bridges between renal ischemia-reperfusion injury and T cell-mediated immune rejection in kidney transplantation.
PLoS One
December 2024
Department of Medical Records Management and Statistics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Unlabelled: Although the link between ischemia-reperfusion injury (IRI) and T cell-mediated rejection (TCMR) in kidney transplantation (KT) is well known, the mechanism remains unclear. We investigated essential genes and biological processes involved in interactions between IRI and TCMR.
Methods: Renal IRI and TCMR datasets were obtained from the Gene Expression Omnibus database.
[Clinical features and prognosis of children with multiple Takayasu's arteritis complicated with carotid artery occlusion].
Zhonghua Yu Fang Yi Xue Za Zhi
December 2024
Children's Hospital Affiliated to Capital Institute of Pediatrics, Beijing100020, China.
This study aimed to analyze the clinical characteristics and prognosis of Takayasu's arteritis (TA) with carotid artery occlusion in children. This study collected clinical data and follow-up information on the first diagnosis and treatment of c-TA combined with carotid artery occlusion in pediatric patients at the Children's Hospital affiliated with the Capital Institute of Pediatrics and Inner Mongolia Medical University Affiliated Hospital from 2013 to 2023. This study included four female patients with a mean age of (13.
View Article and Find Full Text PDFDeletion of lymphotoxin-β receptor (LTβR) protects against acute kidney injury by PPARα pathway.
Mol Med
December 2024
Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, No.1095 Jiefang Road, Wuhan, 430030, China.
Background: Recent data has shown a considerable advancement in understanding the role of lymphotoxin-β receptor (LTβR) in inflammation. However, the functions and underlying mechanisms of LTβR in acute kidney injury (AKI) remain largely unknown.
Methods: AKI was induced in mice by renal ischemia-reperfusion (I/R).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!