Neurobiological evidence suggests that the ketone metabolite β-hydroxybutyrate (BHBA) exerts many neuroprotective functions for the brain. The previous study revealed that BHBA could promote the expression of brain-derived neurotrophic factor (BDNF) at glucose inadequate condition. Here we demonstrated that BHBA administration induced the expression of BDNF in the hippocampus of mice fed with normal diet. In vitro experiment results also showed that 0.02-2 mM BHBA significantly increased BDNF expression in both the primary hippocampal neurons and the hippocampus neuron cell line HT22 under adequate glucose supply. Bdnf transcription induced by BHBA stimulus was mediated through the cAMP/PKA-triggered phosphorylation of CREB (S133) and the subsequent up-regulation of histone H3 Lysine 27 acetylation (H3K27ac) binding at Bdnf promoters I, II, IV, and VI. Moreover, BHBA stimulus induced a decrease in tri-methylation of H3K27 (H3K27me3) binding at the Bdnf promoters II and VI and the elevation of H3K27me3-specific demethylase JMJD3, which also contributed to the activation of Bdnf transcription. These results demonstrated that BHBA within the physiological range could promote BDNF expression in neurons via a novel signaling function. Moreover, BHBA might possess more broad epigenetic regulatory activities, which affected both the acetylation and demethylation of H3K27. Our findings reinforce the beneficial effect of BHBA on the central nervous system (CNS) and suggest that BHBA administration with no need for energy restriction might also be a promising intervention to improve the neuronal activity and ameliorate the degeneration of CNS.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/j.neuroscience.2018.06.036 | DOI Listing |
Vet J
December 2024
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
During the periparturient period, dairy cows experience negative energy balance due to reduced feed intake, leading to adipose tissue breakdown, liver damage, and fat accumulation. This study examined the gut-liver-brain axis to explore the link between fatty liver disease, changes in hypothalamic appetite-related neurons, and microbiome shifts in dairy cows. Thirty cows were monitored, with daily DMI recordings and blood sampling.
View Article and Find Full Text PDFObjective: To evaluate the effect of fasting duration and diet types (plant-based and crickets) on plasma glucose, uric acid, cholesterol, triglycerides, nonesterified fatty acid, β-hydroxybutyric acid (BHBA), and bile acids in bearded dragons after a single feeding.
Methods: Hepatic fat content was estimated by a CT scan. Animals were randomly divided into 2 groups, each receiving either a plant-based or cricket diet.
J Dairy Sci
December 2024
College of Veterinary Medicine, China Agricultural University, 100193, Beijing, China. Electronic address:
Subclinical ketosis (SCK) is highly prevalent and easily overlooked, with insidious and slow progression of hepatic injury, often characterized by an imbalance in immune homeostasis. In nonruminants, macrophage polarization plays an important regulatory role in hepatic lipid accumulation, fibrosis and inflammatory processes. Thus, we aimed to investigate the status of hepatic macrophage polarization in SCK cows and to corroborate its association with liver injury and inflammation.
View Article and Find Full Text PDFAnim Nutr
December 2024
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan 528225, China.
Comput Biol Med
January 2025
Electrical Engineering Department, Amirkabir University of Technology, No. 350, Hafez Ave, Valiasr Square, 15875-4413, Tehran, 159163-4311, Iran. Electronic address:
Cancer, a pervasive and devastating disease, remains a leading global cause of mortality, emphasizing the growing urgency for effective detection methods. Gene Expression Microarray (GEM) data has emerged as a crucial tool in this context, offering insights into early cancer detection and treatment. While deep learning methods offer promise in detecting various cancers through GEM analysis, they suffer from high dimensionality inherent in gene sequences, preventing optimal detection performance across diverse cancer types.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!