Bacterial conjugation, such as that mediated by the E. coli F plasmid, is a main mechanism driving bacterial evolution. Two important proteins required for F-pilus assembly and DNA transfer proficiency are TraW and TrbC. As members of a larger complex, these proteins assemble into a type IV secretion system and are essential components of pore formation and mating pair stabilization between the donor and the recipient cells. In the current report, we demonstrate the physical interaction of TraW and TrbC, show that TraW preferentially interacts with the N-terminal domain of TrbC, and that this interaction is important in restoring conjugation in traW/trbC knockouts.
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Contributions of F-specific subunits to the F plasmid-encoded type IV secretion system and F pilus.
Mol Microbiol
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Department of Microbiology and Molecular Genetics, McGovern Medical School, Houston, Texas, USA.
F plasmids circulate widely among the Enterobacteriaceae through encoded type IV secretion systems (T4SS s). Assembly of T4SS s and associated F pili requires 10 VirB/VirD4-like Tra subunits and eight or more F-specific subunits. Recently, we presented evidence using in situ cryoelectron tomography (cryoET) that T4SS s undergo structural transitions when activated for pilus production, and that assembled pili are deposited onto alternative basal platforms at the cell surface.
View Article and Find Full Text PDFThe interaction of TraW and TrbC is required to facilitate conjugation in F-like plasmids.
Biochem Biophys Res Commun
September 2018
Department of Chemistry & Centre for Research on Biomolecular Interactions, York University, 4700 Keele St., Toronto, ON, M3J 1P3, Canada. Electronic address:
Bacterial conjugation, such as that mediated by the E. coli F plasmid, is a main mechanism driving bacterial evolution. Two important proteins required for F-pilus assembly and DNA transfer proficiency are TraW and TrbC.
View Article and Find Full Text PDFA genomic island present along the bacterial chromosome of the Parachlamydiaceae UWE25, an obligate amoebal endosymbiont, encodes a potentially functional F-like conjugative DNA transfer system.
BMC Microbiol
December 2004
Center for Research on Intracellular Bacteria, Institute of Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
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Department of Medical Microbiology and Immunology, Texas A&M University, College Station 77840.
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