[Effect of PFOA on Oxidative Stress and Membrane Damage of ].

Perfluorooctanoic acid (PFOA) is widely used in industrial production because of its strong chemical stabilities and good hydrophobic and oleophobic properties. It was considered to be a widespread persistent organic pollutant in environment in recent years. The oxidative stress and membrane damage of exposed to PFOA were measured by flow cytometry (FCM) and the toxic mechanism of PFOA was also preliminarily explored. The results showed that, under the stress of PFOA, the intracellular reactive oxygen species (ROS) content of increased, the unsaturation degree of fatty acid decreased, the malondialdehyde (MDA) content increased, the membrane permeability increased, the membrane potential decreased, and the activities of NaK-ATPase and CaMg-ATPase showed a compensatory increase first and then decreased. Therefore, owing to the stress of PFOA, the higher intracellular ROS in reacted with membrane unsaturated fatty acids by peroxidation,and then reduced cell membrane fatty acid saturation, accumulated MDA in cells, and further caused damage to cell membrane, reduced the ATPase activity, and eventually resulted in inactivation or apoptosis of . This study provided more evidence for the further study on environmental ecological toxicology of PFOA.