Ubiquitination plays key roles in eukaryotic growth, stress adaptation, and metabolic regulation. In our previous work, ubiquitin was found to be secreted in the hypovirus-infected strain of , a phytopathogenic filamentous fungus responsible for the chestnut blight. Here we report the functional and molecular characterization of a polyubiquitin gene, , in . The expression of was upregulated by the infection of a hypovirus. Deletion of resulted in abnormal morphology, reduced sporulation, attenuation of virulence, and significant reduction in ubiquitination. A total of 378 sites in 236 proteins were identified to be significantly decreased in ubiquitination in the absence of . Quantitative proteome analysis revealed that 285 in 4,776 identified proteins changed in abundance (1.5-fold, < 0.05) in the null mutant, as compared with the wild-type strain.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6013588 | PMC |
| http://dx.doi.org/10.3389/fmicb.2018.01286 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
mA demethylase CpALKBH regulates mRNA stability to modulate the development and virulence of chestnut blight fungus.
mBio
January 2025
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, China.
Unlabelled: As the most abundant eukaryotic mRNA modification, N-methyladenosine (mA) plays a crucial role in regulating multiple biological processes. This methylation is regulated by methyltransferases and demethylases. However, the regulatory role and mode of action of mA demethylases in fungi remain poorly understood.
View Article and Find Full Text PDFTransmission of Cryphonectria Hypovirus 1 (CHV1) to and In Vitro and In Vivo Testing of Its Potential for Use as Biocontrol Against .
Int J Mol Sci
November 2024
Forest Research, Plant Pathology Department, Alice Holt Lodge, Wrecclesham GU104LH, Surrey, UK.
Cryphonectria hypovirus 1 (CHV1) is successful in controlling , the causal agent of chestnut blight, but little is known regarding its transmission to other fungi, for example the European . In this study, CHV1 was transmitted (circa 200,000-800,000 copies/microliter) to seven isolates from infected . Reverse transmission to virus-free (European 74 testers collection) was achieved, although it was less successful (250-55,000 copies/µL) and was dependent on the vegetative compatibility (VC) group.
View Article and Find Full Text PDFFloristic changes following the chestnut blight may be delayed for decades.
PLoS One
October 2024
Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, CO, United States of America.
Article Synopsis
- A 1920s survey showed chestnuts and oaks were dominant in the White Oak Canyon, Shenandoah National Park, Virginia, but by 1977, chestnuts began to be replaced by other tree species due to chestnut blight.
- A 2021 resurvey revealed significant changes in forest composition, with increased birch and maple, a decline in hemlock and oak, and a notable reduction in chestnut sprouts since 1977, likely due to repeated diebacks affecting their root systems.
- The study indicates that the long-term ecological impact of losing American chestnuts has been more profound than previously understood, highlighting a shift toward early to mid-successional tree species in the forest ecosystem.
New Detection Methods for Cryphonectria Hypovirus 1 (CHV1) through SYBR Green-Based Real-Time PCR and Loop-Mediated Isothermal Amplification (LAMP).
Viruses
July 2024
Department of Plant Protection, Faculty of Agriculture, Bolu Abant İzzet Baysal University, Bolu 14030, Türkiye.
Some mycoviruses can be considered as effective biocontrol agents, mitigating the impact of phytopathogenic fungi and consequently reducing disease outbreaks while promoting plant health. , the causal agent of chestnut blight and a highly destructive pathogen, experienced a notable decrease in its virulence with the identification of cryphonectria hypovirus 1 (CHV1), a naturally occurring biocontrol agent. In this study, two innovative diagnostic protocols designed for the accurate and efficient detection of CHV1 are introduced.
View Article and Find Full Text PDFN6-methyladenosine RNA methyltransferase CpMTA1 mediates CpAphA mRNA stability through a YTHDF1-dependent m6A modification in the chestnut blight fungus.
PLoS Pathog
August 2024
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, China.
In eukaryotic cells, N6-methyladenosine (m6A) is the most prevalent RNA epigenetic modification that plays crucial roles in multiple biological processes. Nevertheless, the functions and regulatory mechanisms of m6A in phytopathogenic fungi are poorly understood. Here, we showed that CpMTA1, an m6A methyltransferase in Cryphonectria parasitica, plays a crucial role in fungal phenotypic traits, virulence, and stress tolerance.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!